понедельник, 18 апреля 2011 г.

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IJMS, Vol. 12, Pages 2631-2640: Assessment of Acetylcholinesterase Activity Using Indoxylacetate and Comparison with the Standard Ellman’s Method

Posted: 18 Apr 2011 12:00 AM PDT

Assay of acetylcholinesterase (AChE) activity plays an important role in diagnostic, detection of pesticides and nerve agents, in vitro characterization of toxins and drugs including potential treatments for Alzheimer's disease. These experiments were done in order to determine whether indoxylacetate could be an adequate chromogenic reactant for AChE assay evaluation. Moreover, the results were compared to the standard Ellman's method. We calculated Michaelis constant Km (2.06 × 10−4 mol/L for acetylthiocholine and 3.21 × 10−3 mol/L for indoxylacetate) maximum reaction velocity Vmax (4.97 × 10−7 kat for acetylcholine and 7.71 × 10−8 kat for indoxylacetate) for electric eel AChE. In a second part, inhibition values were plotted for paraoxon, and reactivation efficacy was measured for some standard oxime reactivators: obidoxime, pralidoxime (2-PAM) and HI-6. Though indoxylacetate is split with lower turnover rate, this compound appears as a very attractive reactant since it does not show any chemical reactivity with oxime antidots and thiol used for the Ellman's method. Thus it can be advantageously used for accurate measurement of AChE activity. Suitability of assay for butyrylcholinesterase activity assessment is also discussed.

IJMS, Vol. 12, Pages 2618-2630: Antioxidant Effects of the Ethanol Extract from Flower of Camellia japonica via Scavenging of Reactive Oxygen Species and Induction of Antioxidant Enzymes

Posted: 18 Apr 2011 12:00 AM PDT

The aim of this study was to investigate the antioxidant properties of the ethanol extract of the flower of Camellia japonica (Camellia extract). Camellia extract exhibited 1,1-diphenyl-2-picrylhydrazyl radical and intracellular reactive oxygen species (ROS) scavenging activity in human HaCaT keratinocytes. In addition, Camellia extract scavenged superoxide anion generated by xanthine/xanthine oxidase and hydroxyl radical generated by the Fenton reaction (FeSO4 + H2O2) in a cell-free system, which was detected by electron spin resonance spectrometry. Furthermore, Camellia extract increased the protein expressions and activity of cellular antioxidant enzymes, such as superoxide dismutase, catalase and glutathione peroxidase. These results suggest that Camellia extract exhibits antioxidant properties by scavenging ROS and enhancing antioxidant enzymes. Camellia extract contained quercetin, quercetin-3-O-glucoside, quercitrin and kaempferol, which are antioxidant compounds.

IJMS, Vol. 12, Pages 2598-2617: Effect of Temperature on the Physico-Chemical Properties of a Room Temperature Ionic Liquid (1-Methyl-3-pentylimidazolium Hexafluorophosphate) with Polyethylene Glycol Oligomer

Posted: 18 Apr 2011 12:00 AM PDT

A systematic study of the effect of composition on the thermo-physical properties of the binary mixtures of 1-methyl-3-pentyl imidazolium hexafluorophosphate [MPI][PF6] with poly(ethylene glycol) (PEG) [Mw = 400] is presented. The excess molar volume, refractive index deviation, viscosity deviation, and surface tension deviation values were calculated from these experimental density, ρ, refractive index, n, viscosity, η, and surface tension, γ, over the whole concentration range, respectively. The excess molar volumes are negative and continue to become increasingly negative with increasing temperature; whereas the viscosity and surface tension deviation are negative and become less negative with increasing temperature. The surface thermodynamic functions, such as surface entropy, enthalpy, as well as standard molar entropy, Parachor, and molar enthalpy of vaporization for pure ionic liquid, have been derived from the temperature dependence of the surface tension values.

IJERPH, Vol. 8, Pages 1206-1221: Combining a Fuzzy Matter-Element Model with a Geographic Information System in Eco-Environmental Sensitivity and Distribution of Land Use Planning

Posted: 18 Apr 2011 12:00 AM PDT

Sustainable ecological and environmental development is the basis of regional development. The sensitivity classification of the ecological environment is the premise of its spatial distribution for land use planning. In this paper, a fuzzy matter-element model and factor-overlay method were employed to analyze the ecological sensitivity in Yicheng City. Four ecological indicators, including soil condition,, water condition,, atmospheric conditions and biodiversity were used to classify the ecological sensitivity. The results were categorized into five ranks: insensitive, slightly sensitive, moderately sensitive, highly sensitive and extremely sensitive zones. The spatial distribution map of environmental sensitivity for land use planning was obtained using GIS (Geographical Information System) techniques. The results illustrated that the extremely sensitive and highly sensitive areas accounted for 14.40% and 30.12% of the total area, respectively, while the moderately sensitive and slightly sensitive areas are 25.99% and 29.49%, respectively. The results provide the theoretical foundation for land use planning by categorizing all kinds of land types in Yicheng City.

Sensors, Vol. 11, Pages 4462-4473: Immunity-Based Diagnosis for a Motherboard

Posted: 18 Apr 2011 12:00 AM PDT

We have utilized immunity-based diagnosis to detect abnormal behavior of components on a motherboard. The immunity-based diagnostic model monitors voltages of some components, CPU temperatures, and fan speeds. We simulated abnormal behaviors of some components on the motherboard, and we utilized the immunity-based diagnostic model to evaluate motherboard sensors in two experiments. These experiments showed that the immunity-based diagnostic model was an effective method for detecting abnormal behavior of components on the motherboard.

Sensors, Vol. 11, Pages 4438-4461: Distributed Dynamic Host Configuration Protocol (D2HCP)

Posted: 18 Apr 2011 12:00 AM PDT

Mobile Ad Hoc Networks (MANETs) are multihop wireless networks of mobile nodes without any fixed or preexisting infrastructure. The topology of these networks can change randomly due to the unpredictable mobility of nodes and their propagation characteristics. In most networks, including MANETs, each node needs a unique identifier to communicate. This work presents a distributed protocol for dynamic node IP address assignment in MANETs. Nodes of a MANET synchronize from time to time to maintain a record of IP address assignments in the entire network and detect any IP address leaks. The proposed stateful autoconfiguration scheme uses the OLSR proactive routing protocol for synchronization and guarantees unique IP addresses under a variety of network conditions, including message losses and network partitioning. Simulation results show that the protocol incurs low latency and communication overhead for IP address assignment.

Duke Nukem Forever - PC - Screenshots

Posted: 18 Apr 2011 03:24 AM PDT

Fact sheet pending.

Papa Penguin - iPhone - Screenshots

Posted: 18 Apr 2011 03:24 AM PDT

Help Papa Penguin look after his children!
• Collect the fish and take it to your little kids!
• Be smart! It's not an easy task to get to the most delicious fish.
• Don't forget about the oxygen! You have to plan your route to get all the fish to the surface!
• Beware of poisonous jelly-fish and sharp-toothed sea raptors!
• Enjoy your antarctic diving with the beautiful ambient underwater music.

The Relic - iPhone - Screenshots

Posted: 18 Apr 2011 03:24 AM PDT

The Relic mixes modern gameplay and relentless arcade action with classic top down visuals!
HACK n SLASH: Use your battle-axe to scythe through the waves of enemies that attack, pick up special power ups along the way for those tougher foes!
EPIC ADVENTURE: Traverse across a wide variety of dungeons in your quest; try not to get lost in the labyrinths!
GAIN EXPERIENCE: Collect kills to buy upgrades, grow more powerful and make the evil forces pay! Share scores and achievements online with Chillingo's Crystal Social Gaming Platform.

Game War : 18/04/2011

Posted: 03 Jan 2011 03:09 PM PST


War

Game Find Words : 18/04/2011

Posted: 03 Jan 2011 05:00 PM PST


Find Words

Game South america geography : 18/04/2011

Posted: 04 Dec 2010 12:09 AM PST


South america geography

Game Substraction action : 18/04/2011

Posted: 03 Jan 2011 04:09 PM PST


Substraction action

Game Hitch cock : 18/04/2011

Posted: 04 Dec 2010 12:07 AM PST


Hitch cock

Game Dr bob : 18/04/2011

Posted: 03 Jan 2011 03:07 PM PST


Dr bob

Game Celebrity simon : 18/04/2011

Posted: 03 Jan 2011 04:09 PM PST


Celebrity simon

Game In the land of the pumpkin : 18/04/2011

Posted: 03 Jan 2011 03:03 PM PST


In the land of the pumpkin

Game Fly bat : 18/04/2011

Posted: 03 Jan 2011 04:04 PM PST


Fly bat

Game Chicken egg : 18/04/2011

Posted: 04 Dec 2010 12:05 AM PST


Chicken egg

Game Africa geography : 18/04/2011

Posted: 04 Dec 2010 12:01 AM PST


Africa geography

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