Games |
- Uprising 44 Screens
- IJMS, Vol. 13, Pages 534-539: A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China
- IJMS, Vol. 13, Pages 516-533: Biodegradable Nanoparticles of mPEG-PLGA-PLL Triblock Copolymers as Novel Non-Viral Vectors for Improving siRNA Delivery and Gene Silencing
- IJMS, Vol. 13, Pages 506-515: Molecular Cloning and Functional Characterization of Tibetan Porcine STING
- IJMS, Vol. 13, Pages 491-505: Occurrence and Biodegradation of Nonylphenol in the Environment
- IJMS, Vol. 13, Pages 477-490: Non Coding RNAs and Viruses in the Framework of the Phylogeny of the Genes, Epigenesis and Heredity
- Future Internet, Vol. 4, Pages 42-64: Extension Activity Support System (EASY): A Web-Based Prototype for Facilitating Farm Management
- Remote Sensing, Vol. 4, Pages 68-87: Identifying Spatial Units of Human Occupation in the Brazilian Amazon Using Landsat and CBERS Multi-Resolution Imagery
- Remote Sensing, Vol. 4, Pages 43-67: Measurement of Surface Displacement and Deformation of Mass Movements Using Least Squares Matching of Repeat High Resolution Satellite and Aerial Images
- Information, Vol. 3, Pages 16-20: Introduction to the Special Issue on Information: Selected Papers from “FIS 2010 Beijing”
- AC Expanding Gambling, Drinking Options in Casinos
- Information, Vol. 3, Pages 1-15: Self-Organized Complexity and Coherent Infomax from the Viewpoint of Jaynes’s Probability Theory
- Molecules, Vol. 17, Pages 452-462: A Biosorption Isotherm Model for the Removal of Reactive Azo Dyes by Inactivated Mycelia of Cunninghamella elegans UCP542
- Speed Download 5.2.29 - Versatile download manager and accelerator.. (Shareware)
- X-Plane 10.03b8 - Ultra-realistic flight simulator.. (Updater)
- Molecules, Vol. 17, Pages 433-451: Synthesis of Stable and Soluble One-Handed Helical Homopoly(substituted acetylene)s without the Coexistence of Any Other Chiral Moieties via Two-Step Polymer Reactions in Membrane State: Molecular Design of the Starting Monomer
- Molecules, Vol. 17, Pages 420-432: Metabolite Profiling of Four Major Flavonoids of Herba Epimdii in Zebrafish
- Molecules, Vol. 17, Pages 408-419: Extraction Equilibrium of Indium(III) from Nitric Acid Solutions by Di(2-ethylhexyl)phosphoric Acid Dissolved in Kerosene
- Molecules, Vol. 17, Pages 390-407: Variations of Antioxidant Characteristics and Mineral Contents in Pulp and Peel of Different Apple (Malus domestica Borkh.) Cultivars from Pakistan
- Powerball Winning Numbers for 12/31/2011
| Posted: 03 Jan 2012 03:30 PM PST 35 new shots posted.
Get the full article at GameSpot "Uprising 44 Screens" was posted on Tue, 03 Jan 2012 15:30:00 -0800 |
| Posted: 04 Jan 2012 12:00 AM PST The genus Luculia Sweet contains about five species of small trees or shrubs and is a member of the family Rubiaceae (tribe Cinchoneae). Luculia yunnanensis is an endangered ornamental shrub endemic to southwest China. Only two natural populations of L. yunnanensis exist in the wild according to our field investigation. It can be inferred that L. yunnanensis is facing a very high risk of extinction in the wild and an urgent conservation strategy is required. By using a modified biotin-sterptavidin capture method, 24 primer sets were identified in two wild populations. Of these primers, 11 displayed polymorphisms and 13 were monomorphic. The number of alleles per locus ranged from two to four, values for observed and expected heterozygosities ranged from 0.000 to 0.833 and from 0.431 to 0.771, with averages of 0.389 and 0.614, respectively. These markers will be useful for further investigation of conservation of resources, selecting parental types in cross-breeding, evolution of this species at the molecular level and related research in Luculia species. |
| Posted: 04 Jan 2012 12:00 AM PST Degradation of mRNA by RNA interference is one of the most powerful and specific mechanisms for gene silencing. However, insufficient cellular uptake and poor stability have limited its usefulness. Here, we report efficient delivery of siRNA via the use of biodegradable nanoparticles (NPs) made from monomethoxypoly(ethylene glycol)-poly(lactic-co-glycolic acid)-poly-l-lysine (mPEG-PLGA-PLL) triblock copolymers. Various physicochemical properties of mPEG-PLGA-PLL NPs, including morphology, size, surface charge, siRNA encapsulation efficiency, and in vitro release profile of siRNA from NPs, were characterized by scanning electron microscope, particle size and zeta potential analyzer, and high performance liquid chromatography. The levels of siRNA uptake and targeted gene inhibition were detected in human lung cancer SPC-A1-GFP cells stably expressing green fluorescent protein. Examination of the cultured SPC-A1-GFP cells with fluorescent microscope and flow cytometry showed NPs loading Cy3-labeled siRNA had much higher intracellular siRNA delivery efficiencies than siRNA alone and Lipofectamine-siRNA complexes. The gene silencing efficiency of mPEG-PLGA-PLL NPs was higher than that of commercially available transfecting agent Lipofectamine while showing no cytotoxicity. Thus, the current study demonstrates that biodegradable NPs of mPEG-PLGA-PLL triblock copolymers can be potentially applied as novel non-viral vectors for improving siRNA delivery and gene silencing. |
| Posted: 04 Jan 2012 12:00 AM PST Tibetan pig is well known for its strong disease resistance. However, little is known about the molecular basis of its strong resistance to disease. Stimulator of interferon (IFN) genes (STING), also known as MPYS/MITA/ERIS/TMEM173, is an adaptor that functions downstream of RIG-I and MAVS and upstream of TBK1 and plays a critical role in type I IFN induction. Here we report the first cloning and characterization of STING gene from Tibetan pig. The entire open reading frame (ORF) of the Tibetan porcine STING is 1137 bp, with a higher degree of sequence similarity with Landrace pig (98%) and cattle (88%) than with chimpanzee (84%), human (83%) or mouse (77%). The predicted protein is composed of 378 amino acids and has 4 putative transmembrane domains. Real-time quantitative PCR analysis indicated that Tibetan pig STING mRNA was most abundant in the lung and heart. Overexpression of Tibetan porcine STING led to upregulation of IFN-β and IFN-stimulated gene 15 (ISG15) in porcine jejunal epithelial cell line IPEC-J2 cells. This is the first study investigating the biological role of STING in intestinal epithelial cells, which lays a foundation for the further study of STING in intestinal innate immunity. |
| IJMS, Vol. 13, Pages 491-505: Occurrence and Biodegradation of Nonylphenol in the Environment Posted: 04 Jan 2012 12:00 AM PST Nonylphenol (NP) is an ultimate degradation product of nonylphenol polyethoxylates (NPE) that is primarily used in cleaning and industrial processes. Its widespread use has led to the wide existence of NP in various environmental matrices, such as water, sediment, air and soil. NP can be decreased by biodegradation through the action of microorganisms under aerobic or anaerobic conditions. Half-lives of biodegradation ranged from a few days to almost one hundred days. The degradation rate for NP was influenced by temperature, pH and additions of yeast extracts, surfactants, aluminum sulfate, acetate, pyruvate, lactate, manganese dioxide, ferric chloride, sodium chloride, hydrogen peroxide, heavy metals, and phthalic acid esters. Although NP is present at low concentrations in the environment, as an endocrine disruptor the risks of long-term exposure to low concentrations remain largely unknown. This paper reviews the occurrence of NP in the environment and its aerobic and anaerobic biodegradation in natural environments and sewage treatment plants, which is essential for assessing the potential risk associated with low level exposure to NP and other endocrine disruptors. |
| Posted: 04 Jan 2012 12:00 AM PST The origin of genes is one of the most enigmatic events in the origin of life. It has been suggested that noncoding (nc) RNA was probably a precursor in the formation of the first polypeptide, and also at the origin of the first manifestation of life and genes. ncRNAs are also becoming central for understanding gene expression and silencing. Indeed, before the discovery of ncRNAs, proteins were viewed as the major molecules in the regulation of gene expression and gene silencing; however, recent findings suggest that ncRNA also plays an important role in gene expression. Reverse transcription of RNA viruses and their integration into the genome of eukaryotes and also their relationship with the ncRNA suggest that their origin is basal in genome evolution, and also probably constitute the first mechanism of gene regulation. I am to review the different roles of ncRNAs in the framework of gene evolution, as well as the importance of ncRNAs and viruses in the epigenesis and in the non-Mendelian model of heredity and evolution. |
| Posted: 04 Jan 2012 12:00 AM PST In response to disparate advances in delivering spatial information to support agricultural extension activities, the Extension Activity Support System (EASY) project was established to develop a vision statement and conceptual design for such a system based on a national needs assessment. Personnel from across Australia were consulted and a review of existing farm information/management software undertaken to ensure that any system that is eventually produced from the EASY vision will build on the strengths of existing efforts. This paper reports on the collaborative consultative process undertaken to create the EASY vision as well as the conceptual technical design and business models that could support a fully functional spatially enabled online system. |
| Posted: 04 Jan 2012 12:00 AM PST Every spatial unit of human occupation is part of a network structuring an extensive process of urbanization in the Amazon territory. Multi-resolution remote sensing data were used to identify and map human presence and activities in the Sustainable Forest District of Cuiabá-Santarém highway (BR-163), west of Pará, Brazil. The limits of spatial units of human occupation were mapped based on digital classification of Landsat-TM5 (Thematic Mapper 5) image (30m spatial resolution). High-spatial-resolution CBERS-HRC (China-Brazil Earth Resources Satellite-High-Resolution Camera) images (5 m) merged with CBERS-CCD (Charge Coupled Device) images (20 m) were used to map spatial arrangements inside each populated unit, describing intra-urban characteristics. Fieldwork data validated and refined the classification maps that supported the categorization of the units. A total of 133 spatial units were individualized, comprising population centers as municipal seats, villages and communities, and units of human activities, such as sawmills, farmhouses, landing strips, etc. From the high-resolution analysis, 32 population centers were grouped in four categories, described according to their level of urbanization and spatial organization as: structured, recent, established and dependent on connectivity. This multi-resolution approach provided spatial information about the urbanization process and organization of the territory. It may be extended into other areas or be further used to devise a monitoring system, contributing to the discussion of public policy priorities for sustainable development in the Amazon. |
| Posted: 04 Jan 2012 12:00 AM PST Displacement and deformation are fundamental measures of Earth surface mass movements such as glacier flow, rockglacier creep and rockslides. Ground-based methods of monitoring such mass movements can be costly, time consuming and limited in spatial and temporal coverage. Remote sensing techniques, here matching of repeat optical images, are increasingly used to obtain displacement and deformation fields. Strain rates are usually computed in a post-processing step based on the gradients of the measured velocity field. This study explores the potential of automatically and directly computing velocity, rotation and strain rates on Earth surface mass movements simultaneously from the matching positions and the parameters of the geometric transformation models using the least squares matching (LSM) approach. The procedures are exemplified using bi-temporal high resolution satellite and aerial images of glacier flow, rockglacier creep and land sliding. The results show that LSM matches the images and computes longitudinal strain rates, transverse strain rates and shear strain rates reliably with mean absolute deviations in the order of 10−4 (one level of significance below the measured values) as evaluated on stable grounds. The LSM also improves the accuracy of displacement estimation of the pixel-precision normalized cross-correlation by over 90% under ideal (simulated) circumstances and by about 25% for real multi-temporal images of mass movements. |
| Posted: 04 Jan 2012 12:00 AM PST During the last two decades, a systematic re-examination of the whole information science field has taken place around the FIS—Foundations of Information Science—initiative. With the occasion of its Fourth Conference in Beijing 2010, a group of selected contributors and leading practitioners of those fields have been invited to contribute to this Special Issue. What is the status of information science today? What is the relationship between information and the laws of nature? Is information merely "physical"? What is the difference between information and computation? Has the genomic revolution changed the contemporary views on information and life? And what about the nature of social information? Cogent answers to these questions and to quite many others are attempted in the contributions that follow. |
| AC Expanding Gambling, Drinking Options in Casinos Posted: 03 Jan 2012 11:47 PM PST New Jersey regulators are letting casinos put slot machines and table games into places they've never been before. This posting includes an audio/video/photo media file: Download Now |
| Posted: 04 Jan 2012 12:00 AM PST This paper discusses concepts of self-organized complexity and the theory of Coherent Infomax in the light of Jaynes's probability theory. Coherent Infomax, shows, in principle, how adaptively self-organized complexity can be preserved and improved by using probabilistic inference that is context-sensitive. It argues that neural systems do this by combining local reliability with flexible, holistic, context-sensitivity. Jaynes argued that the logic of probabilistic inference shows it to be based upon Bayesian and Maximum Entropy methods or special cases of them. He presented his probability theory as the logic of science; here it is considered as the logic of life. It is concluded that the theory of Coherent Infomax specifies a general objective for probabilistic inference, and that contextual interactions in neural systems perform functions required of the scientist within Jaynes's theory. |
| Posted: 04 Jan 2012 12:00 AM PST The biosorption of three reactive azo dyes (red, black and orange II) found in textile effluents by inactive mycelium of Cunninghamella elegans has been investigated. It was found that after 120 hours of contact the adsorption led to 70%, 85%, 93% and 88% removal of reactive orange II, reactive black, reactive red and a mixture of them, respectively. The mycelium surface was found to be selective towards the azo dyes in the following order: reactive red > reactive black > orange II. Dye removal from a mixture solution resulted in 48.4 mg/g retention by mycelium and indicated a competition amongst the dyes for the cellular surface. A Freundlich adsorption isotherm model exhibited a better fit, thus suggesting the presence of heterogeneous binding sites. Electrondense deposits observed on the mycelium ultrastructure suggest that the dyes are mainly retained under the cellular surface of the inactive biomass of C. elegans. |
| Speed Download 5.2.29 - Versatile download manager and accelerator.. (Shareware) Posted: 03 Jan 2012 08:59 PM PST  Speed Download combines four amazing products into one small and easy to use application:
Key features:
Version 5.2.29:
Mac OS X 10.5.8 or later Download Now This posting includes an audio/video/photo media file: Download Now |
| X-Plane 10.03b8 - Ultra-realistic flight simulator.. (Updater) Posted: 03 Jan 2012 08:59 PM PST  X-Plane is the world's most comprehensive, powerful flight simulator, and has the most realistic flight model available for personal computers. Welcome to the world of props, jets, single- and multi-engine airplanes, as well as gliders, helicopters and VTOLs such as the V-22 Osprey and AV8-B Harrier. X-Plane comes with subsonic and supersonic flight dynamics, sporting aircraft from the Bell 206 Jet-Ranger helicopter and Cessna 172 light plane to the supersonic SR-71 and Space Shuttle. X-Plane comes with 35 aircraft spanning the aviation industry (and history), and several thousand more are freely downloadable from the internet. (See www.X-Plane.org as a good place to start). X-Plane scenery is world-wide, with scenery for the entire planet Earth between 74 degrees north to 60 degrees south latitude. We also have MARS scenery! (thanks to the Mars Orbiting Laser Altimeter, which mapped that planet's elevation). On Earth, you can land at any of over 33,000 airports, as well as test your mettle on aircraft carriers, helipads on building tops, frigates that pitch and roll in the waves, and oil rigs. Weather is variable from clear skies and high visibility to thunderstorms with controllable wind, wind shear, turbulence, and microbursts! Rain, snow and clouds are available for an instrument flying challenge, and thermals are available for the gliders! Real weather conditions can be downloaded from the internet, allowing you to fly in the actual weather that currently exists at the location of flight! X-Plane also has detailed failure-modeling, with multitudes of systems that can be failed manually or randomly, when you least expect it! You can fail instruments, engines, flight controls, landing gear, or dozens of other systems at any moment. While X-Plane is the world's most COMPREHENSIVE flight sim, your purchase also comes with Plane-Maker (to create your own airplanes) Airfoil-Maker (allowing users to create airfoil performance profiles), and Weather Briefer (to get a weather briefing before the flight if you use real weather conditions downloaded from the internet). X-Plane is also extremely customizable, allowing you to easily create textures, sounds, and instrument panels for your own airplanes that you design or the planes that come with the sim. X-Plane's accuracy (in flight model), scope (in aircraft and terrain coverage), versatility (in aircraft type and weather conditions), add-on programs (in aircraft and scenery editors), customizability, downloadable aircraft, and downloadable scenery makes it the ULTIMATE flight simulation experience for Macintosh, Windows, and Linux platforms. BE SURE TO TURN ON THE >BETA< option in the installer before you run it to get the beta! The demo contains a limited scenery area to keep the download size reasonable. It will run for 10 minutes before it begins to ignore the user's input, at which point the simulator must be restarted to fly more. Version 10.03b8: Coolness:
Download Now This posting includes an audio/video/photo media file: Download Now |
| Posted: 04 Jan 2012 12:00 AM PST A soluble and stable one-handed helical poly(substituted phenylacetylene) without the coexistence of any other chiral moieties was successfully synthesized by asymmetric-induced polymerization of a chiral monomer followed by two-step polymer reactions in membrane state: (1) removing the chiral groups (desubstitution); and (2) introduction of achiral long alkyl groups at the same position as the desubstitution to enhance the solubility of the resulting one-handed helical polymer (resubstitution). The starting chiral monomer should have four characteristic substituents: (i) a chiral group bonded to an easily hydrolyzed spacer group; (ii) two hydroxyl groups; (iii) a long rigid hydrophobic spacer between the chiral group and the polymerizing group; (iv) a long achiral group near the chiral group. As spacer group a carbonate ester was selected. The two hydroxyl groups formed intramolecular hydrogen bonds stabilizing a one-handed helical structure in solution before and after the two-step polymer reactions in membrane state. The rigid long hydrophobic spacer, a phenylethynylphenyl group, enhanced the solubility of the starting polymer, and realized effective chiral induction from the chiral side groups to the main chain in the asymmetric-induced polymerization. The long alkyl group near the chiral group avoided shrinkage of the membrane and kept the reactivity of resubstitution in membrane state after removing the chiral groups. The g value (g = ([θ]/3,300)/ε) for the CD signal assigned to the main chain in the obtained final polymer was almost the same as that of the starting polymer in spite of the absence of any other chiral moieties. Moreover, since the one-handed helical structure was maintained by the intramolecular hydrogen bonds in a solution, direct observation of the one-handed helicity of the final homopolymer has been realized in CD for the solution for the first time. |
| Posted: 04 Jan 2012 12:00 AM PST The zebrafish model organism was applied first in a metabolic study of icariin, baohuoside I, epimedin A and epimedin C, which are flavonoids in Herba Epimedii. Metabolites of these compounds in zebrafish after exposure for 24 h were identified by HPLC-ESI-MS, whereby the separation was performed with a Zorbax C-18 column using a gradient elution of 0.05% formic acid acetonitrile-0.05% formic acid water. The quasi-molecular ions of compounds were detected in simultaneous negative and positive ionization modes. Metabolic products of icariin and epimedin C via cleavage of glucose residue instead of rhamnose residues were found, which coincided with the results using regular metabolic analysis methods. In addition, the zebrafish model was used to predict the metabolism of the trace component epimedin A, whose metabolic mechanisms haven't been clearly elucidated with the current metabolism model. The metabolic pathway of epimedin A in zebrafish was similar to those of its homologue icariin and epimedin C. Our study demonstrated that the zebrafish model can successfully imitate the current models in elucidating metabolic pathways of model flavonoids, which has advantages of lower cost, far less amount of compound needed, easy set up and high performance. This novel model can also be applied in quickly predicting the metabolism of Chinese herb components, especially trace compounds. |
| Posted: 04 Jan 2012 12:00 AM PST The extraction equilibrium of indium(III) from a nitric acid solution using di(2-ethylhexyl) phosphoric acid (D2EHPA) as an acidic extractant of organophosphorus compounds dissolved in kerosene was studied. By graphical and numerical analysis, the compositions of indium-D2EHPA complexes in organic phase and stoichiometry of the extraction reaction were examined. Nitric acid solutions with various indium concentrations at 25 °C were used to obtain the equilibrium constant of InR3 in the organic phase. The experimental results showed that the extraction distribution ratios of indium(III) between the organic phase and the aqueous solution increased when either the pH value of the aqueous solution and/or the concentration of the organic phase extractant increased. Finally, the recovery efficiency of indium(III) in nitric acid was measured. |
| Posted: 04 Jan 2012 12:00 AM PST Variations of phenolics, antioxidant activity, and mineral contents in peel and pulp of five apple (Malus domestica Borkh.) cultivars from Pakistan, namely Red Delicious, Golden Delicious, Kashmiri Amri, Kala Kulu and Sky Spur were appraised. The mean extract yield of antioxidant components obtained with 80:20 methanol-water (v/v), was found to be 22.1 g/100 g for peel and 14.2 g/100 g for pulp on a dry weight basis. The amounts of total phenolics and total flavonoids in peel and pulp of different cultivars of apple ranged from 1,907.5–2,587.9 mg gallic acid equivalent/100 g DW and 1,214.3–1,816.4 mg catechin equivalent/100 g DW and 1,185.2–1,475.5 mg GAE/100 g DW and 711.8–999.3 mg CE/100 g DW, respectively. The inhibition of linoleic acid peroxidation and DPPH scavenging activity of the extracts varied from 71.7–84.9 and 66.6–80.8% in peel, and 43.9–52.8 and 42.9–51.1% in pulp, respectively. Reducing power of the tested fruit part extracts at concentration 12.5 mg/mL ranged from 2.54–2.89 and 1.37–1.73, respectively. With regard to minerals analysis, both fruit parts showed the amount of K to be the highest, followed by Mg, Ca, Fe, Na and Zn. The results revealed that peel of the tested apple cultivars in this study had superior antioxidant capacity and mineral concentration than the pulp, indicating significant variations between the parts tested. Thus, consumption of apple fruits along with peel might be recommended to gaining better nutritive benefits. |
| Powerball Winning Numbers for 12/31/2011 Posted: 03 Jan 2012 10:15 PM PST 5 - 23 - 25 - 28 - 40 Powerball 34 Power Play 4 |
| You are subscribed to email updates from Games To stop receiving these emails, you may unsubscribe now. | Email delivery powered by Google |
| Google Inc., 20 West Kinzie, Chicago IL USA 60610 | |
Комментариев нет:
Отправить комментарий