Games |
- EA gives away a huge number of free games... by mistake
- Adobe Flash Professional CS6 12.0.2.529 - Advanced authoring environment for creating interactive content.. (Demo)
- IJMS, Vol. 13, Pages 13227-13239: MicroRNAs as Active Players in the Pathogenesis of Multiple Sclerosis
- IJMS, Vol. 13, Pages 13212-13226: Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris
- IJMS, Vol. 13, Pages 13203-13211: Isolation and Characterization of Novel Genomic and EST-SSR Markers in Coreoperca whiteheadi Boulenger and Cross-Species Amplification
- IJMS, Vol. 13, Pages 13183-13202: Altered DNA Binding and Amplification of Human Breast Cancer Suppressor Gene BRCA1 Induced by a Novel Antitumor Compound, [Ru(η6-p-phenylethacrynate)Cl2(pta)]
- IJERPH, Vol. 9, Pages 3654-3684: Implementation of BMP Strategies for Adaptation to Climate Change and Land Use Change in a Pasture-Dominated Watershed
- IJERPH, Vol. 9, Pages 3629-3653: Response of Fish Communities to Various Environmental Variables across Multiple Spatial Scales
- IJERPH, Vol. 9, Pages 3599-3628: A Multimetric Benthic Macroinvertebrate Index for the Assessment of Stream Biotic Integrity in Korea
- Fake 1.8.7 - Browser that simplifies Web automation.. (Shareware)
- Apple Security Update 2012-004 - For Mac OS X 10.6 and 10.6 Server.. (Updater)
- The Last of Us: four-issue Dark Horse comic series and deluxe art book announced
- Viruses, Vol. 4, Pages 2115-2136: A Characterization of Aerosolized Sudan Virus Infection in African Green Monkeys, Cynomolgus Macaques, and Rhesus Macaques
- Pharmaceuticals, Vol. 5, Pages 1092-1102: Intranasal Delivery of Camptothecin-Loaded Tat-Modified Nanomicells for Treatment of Intracranial Brain Tumors
- Nutrients, Vol. 4, Pages 1464-1474: Food Variety at 2 Years of Age is Related to Duration of Breastfeeding
- Minerals, Vol. 2, Pages 272-282: EDEEP—An Innovative Process for Improving the Safety of Mining Equipment
- Golden Joystick Awards 2012: Best Browser/Flash category – who are you voting for?
- Puzzler World Hits DSi And 3DS
- Materials, Vol. 5, Pages 1853-1872: Theory-Guided Materials Design of Multi-Phase Ti-Nb Alloys with Bone-Matching Elastic Properties
- Genes, Vol. 3, Pages 651-670: Identifying and Characterizing Regulatory Sequences in the Human Genome with Chromatin Accessibility Assays
| EA gives away a huge number of free games... by mistake Posted: 15 Oct 2012 04:12 AM PDT Electronic Arts recently ran a survey promotion on its online digital distribution service, Origin. Fill out some questionnaires, get a download code for |
| Posted: 15 Oct 2012 03:40 AM PDT  Adobe Flash Professional CS6 software is a powerful authoring environment for creating animation and multimedia content. Design immersive interactive experiences that present consistently across desktops and multiple devices, including tablets, smartphones, and televisions. See the top new features in Flash Professional CS6 - Find out more about sprite-sheet generation, simulation of mobile Adobe AIR applications, and other features introduced in this latest version of the leading animation software for producing rich multimedia content. Learn more about new support for HTML5 - Download this new extension to transition to creating HTML5-based interactive content. Continue to use the core animation and drawing capabilities of Flash Professional CS6 and export JavaScript to target the CreateJS open source framework. Expand your reach across devices and platforms - Reach Android and iOS devices by targeting the latest Adobe Flash Player and AIR runtimes. Deliver apps with a prepackaged AIR captive runtime for better user experience, and access device-specific capabilities with prebuilt native extensions. Version 12.0.2.529: Fixes
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| Posted: 15 Oct 2012 12:00 AM PDT MicroRNAs (miRNAs) are a recently discovered group of small noncoding RNAs that regulate gene expression post-transcriptionally. They are highly expressed in cells of the immune system, as well as in the central nervous system, and they are deregulated in various neurological disorders. Emerging evidence underlines an involvement of miRNAs in the pathogenesis of Multiple Sclerosis (MS). A number of miRNAs have been found to be dysregulated in blood cells from MS patients, in brain lesions, as well as in biological fluids such as serum and plasma. Despite miRNA altered expression likely showing a high tissue specificity, some profile similarities could be observed for certain miRNAs such as miR-326—such as upregulation in both active lesions and blood—though not for others such as miR-323, which demonstrated upregulation in whole blood, active brain lesions, and T-reg cells, but not in the serum of MS patients. In this review, the possible role of miRNAs in MS pathogenesis will be discussed according to all the available literature, with a particular emphasis on the possibility of considering extracellular miRNAs as a new source for both biomarker identification and therapeutic target discovery. |
| Posted: 15 Oct 2012 12:00 AM PDT An heterologous expression of Vitreoscilla hemoglobin (VHb) for improving cell growth and recombinant protein production has been successfully demonstrated in various hosts, including Pichia pastoris. Lower temperature cultures can enhance target protein production in some studies of P. pastoris. In this study, the strategy of combining heterologous VHb expression and lower temperature cultures in P. pastoris showed that final cell density and viability of VHb+ strain at 23 °C were higher than that at 30 °C. In addition, the effects of VHb expression on recombinant β-galactosidase production and oxygen uptake rate were also higher at 23 °C than at 30 °C. Consequently, lower temperature cultures can enlarge VHb effectiveness on cell performance of P. pastoris. This is because VHb activity obtained at 23 °C cultures was twofold higher than that at 30 °C cultures, due to a different heme production. This strategy makes P. pastoris an excellent expression host particularly suitable for increasing the yields of the low-stability and aggregation-prone recombinant proteins. |
| Posted: 15 Oct 2012 12:00 AM PDT We described and characterized 11 expressed sequence tag (EST)-derived simple sequence repeats (SSR) and seven genomic (G)-derived SSRs in Coreoperca whiteheadi Boulenger. The EST-SSRs comprised 62.2% di-nucleotide repeats, 32.2% tri-nucleotide repeats and 5.5% tetra-nucleotide repeats, whereas the majority of the G-SSRs were tri-nuleotide repeats (81.4%). The number of alleles for the 18 loci ranged from 3 to 6, with a mean of 3.8 alleles per locus. The observed (Ho) and expected heterozygosities (He) values ranged from 0.375 to 1.000, and 0.477 to 0.757, respectively. The polymorphic information content (PIC) values ranged from 0.466 to 0.706. The mean values number of alleles, Ho, He, and PIC of EST-SSRs were higher than those of the G-SSRs. Four microsatellite loci deviated significantly from Hardy-Weinberg equilibrium (HWE) after Bonferroni correction and no significant deviations in linkage disequilibrium (LD) were observed. These loci are the first to be characterized in C. whiteheadi and should be useful in the investigation of a genetic evaluation for conservation. Compared with 11 loci in C. whiteheadi, 37 potential polymorphic EST-SSRs were found in Siniperca chuatsi (Basilewsky), which will provide a valuable tool for mapping studies and molecular breeding programs in S. chuatsi. |
| Posted: 15 Oct 2012 12:00 AM PDT The ruthenium-based complex [Ru(η6-p-phenylethacrynate)Cl2(pta)] (pta = 1,3,5-triaza-7-phosphatricyclo-[3.3.1.1]decane), termed ethaRAPTA, is an interesting antitumor compound. The elucidation of the molecular mechanism of drug activity is central to the drug development program. To this end, we have characterized the ethaRAPTA interaction with DNA, including probing the sequence specific modified DNA structural stability and DNA amplification using the breast cancer suppressor gene 1 (BRCA1) of human breast and colon adenocarcinoma cell lines as models. The preference of ethaRAPTA base binding is in the order A > G > T > C. Once modified, the ethaRAPTA-induced BRCA1 structure has higher thermal stability than the modified equivalents of its related compound, RAPTA-C. EthaRAPTA exhibits a higher efficiency than RAPTA-C in inhibiting BRCA1 amplification. With respect to both compounds, the inhibition of BRCA1 amplification is more effective in an isolated system than in cell lines. These data provide evidence that will help to understand the process of elucidating the pathways involved in the response induced by ethaRAPTA. |
| Posted: 15 Oct 2012 12:00 AM PDT Implementing a suite of best management practices (BMPs) can reduce non-point source (NPS) pollutants from various land use activities. Watershed models are generally used to evaluate the effectiveness of BMP performance in improving water quality as the basis for watershed management recommendations. This study evaluates 171 management practice combinations that incorporate nutrient management, vegetated filter strips (VFS) and grazing management for their performances in improving water quality in a pasture-dominated watershed with dynamic land use changes during 1992–2007 by using the Soil and Water Assessment Tool (SWAT). These selected BMPs were further examined with future climate conditions (2010–2069) downscaled from three general circulation models (GCMs) for understanding how climate change may impact BMP performance. Simulation results indicate that total nitrogen (TN) and total phosphorus (TP) losses increase with increasing litter application rates. Alum-treated litter applications resulted in greater TN losses, and fewer TP losses than the losses from untreated poultry litter applications. For the same litter application rates, sediment and TP losses are greater for summer applications than fall and spring applications, while TN losses are greater for fall applications. Overgrazing management resulted in the greatest sediment and phosphorus losses, and VFS is the most influential management practice in reducing pollutant losses. Simulations also indicate that climate change impacts TSS losses the most, resulting in a larger magnitude of TSS losses. However, the performance of selected BMPs in reducing TN and TP losses was more stable in future climate change conditions than in the BMP performance in the historical climate condition. We recommend that selection of BMPs to reduce TSS losses should be a priority concern when multiple uses of BMPs that benefit nutrient reductions are considered in a watershed. Therefore, the BMP combination of spring litter application, optimum grazing management and filter strip with a VFS ratio of 42 could be a promising alternative for use in mitigating future climate change. |
| Posted: 15 Oct 2012 12:00 AM PDT A better understanding of the relative importance of different spatial scale determinants on fish communities will eventually increase the accuracy and precision of their bioassessments. Many studies have described the influence of environmental variables on fish communities on multiple spatial scales. However, there is very limited information available on this topic for the East Asian monsoon region, including Korea. In this study, we evaluated the relationship between fish communities and environmental variables at multiple spatial scales using self-organizing map (SOM), random forest, and theoretical path models. The SOM explored differences among fish communities, reflecting environmental gradients, such as a longitudinal gradient from upstream to downstream, and differences in land cover types and water quality. The random forest model for predicting fish community patterns that used all 14 environmental variables was more powerful than a model using any single variable or other combination of environmental variables, and the random forest model was effective at predicting the occurrence of species and evaluating the contribution of environmental variables to that prediction. The theoretical path model described the responses of different species to their environment at multiple spatial scales, showing the importance of altitude, forest, and water quality factors to fish assemblages. |
| Posted: 15 Oct 2012 12:00 AM PDT At a time when anthropogenic activities are increasingly disturbing the overall ecological integrity of freshwater ecosystems, monitoring of biological communities is central to assessing the health and function of streams. This study aimed to use a large nation-wide database to develop a multimetric index (the Korean Benthic macroinvertebrate Index of Biological Integrity—KB-IBI) applicable to the biological assessment of Korean streams. Reference and impaired conditions were determined based on watershed, chemical and physical criteria. Eight of an initial 34 candidate metrics were selected using a stepwise procedure that evaluated metric variability, redundancy, sensitivity and responsiveness to environmental gradients. The selected metrics were number of taxa, percent Ephemeroptera-Plecoptera-Trichoptera (EPT) individuals, percent of a dominant taxon, percent taxa abundance without Chironomidae, Shannon’s diversity index, percent gatherer individuals, ratio of filterers and scrapers, and the Korean saprobic index. Our multimetric index successfully distinguished reference from impaired conditions. A scoring system was established for each core metric using its quartile range and response to anthropogenic disturbances. The multimetric index was classified by aggregating the individual metric ..scores and the value range was quadrisected to provide a narrative criterion (Poor, Fair, Good and Excellent) to describe the biological integrity of the streams in the study. A validation procedure showed that the index is an effective method for evaluating stream conditions, and thus is appropriate for use in future studies measuring the long-term status of streams, and the effectiveness of restoration methods. |
| Fake 1.8.7 - Browser that simplifies Web automation.. (Shareware) Posted: 15 Oct 2012 03:00 AM PDT  Fake is a new browser for Mac OS X that makes web automation simple. Fake allows you to drag discrete browser Actions into a graphical Workflow that can be run again and again without human interaction. The Fake Workflows you create can be saved, reopened, and shared. Inspired by Apple's Automator application, Fake looks like a combination of Safari and Automator and allows you to run (and re-run) "fake" interactions with the web. Power Users will love Fake for automating tedious web tasks like filling out lengthy forms and capturing screenshots. Developers can use Fake for graphically configuring automated tests for their webapps, including assertions. All of Fake's automation features are powered by Mac OS X's native scripting tool – AppleScript. Which means Fake can be used to incorporate web automation into many other OS X scripting tasks. Fake's browser component is based on the same open source technology behind the popular Mac OS X Site Specific Browser, Fluid. Fake's proprietary secret sauce is in its web automation capabilities – the Action Library, and Workflow side pane.
Mac OS X 10.5 or later. Download Now This posting includes an audio/video/photo media file: Download Now |
| Apple Security Update 2012-004 - For Mac OS X 10.6 and 10.6 Server.. (Updater) Posted: 15 Oct 2012 03:15 AM PDT Apple Security Update 2012-004 is recommended for all users and improves the security of Mac OS X. For information on the security content of this update, please visit this website: http://support.apple.com/kb/HT1222. This posting includes an audio/video/photo media file: Download Now |
| The Last of Us: four-issue Dark Horse comic series and deluxe art book announced Posted: 15 Oct 2012 03:10 AM PDT Dark Horse comics has announced The Last of Us: American Dreams, a four-issue series penned by The Last of Us lead writer, Neil Druckmann. The comics will |
| Posted: 15 Oct 2012 12:00 AM PDT Filoviruses are members of the genera Ebolavirus, Marburgvirus, and “Cuevavirus”. Because they cause human disease with high lethality and could potentially be used as a bioweapon, these viruses are classified as CDC Category A Bioterrorism Agents. Filoviruses are relatively stable in aerosols, retain virulence after lyophilization, and can be present on contaminated surfaces for extended periods of time. This study explores the characteristics of aerosolized Sudan virus (SUDV) Boniface in non-human primates (NHP) belonging to three different species. Groups of cynomolgus macaques (cyno), rhesus macaques (rhesus), and African green monkeys (AGM) were challenged with target doses of 50 or 500 plaque-forming units (pfu) of aerosolized SUDV. Exposure to either viral dose resulted in increased body temperatures in all three NHP species beginning on days 4–5 post-exposure. Other clinical findings for all three NHP species included leukocytosis, thrombocytopenia, anorexia, dehydration, and lymphadenopathy. Disease in all of the NHPs was severe beginning on day 6 post-exposure, and all animals except one surviving rhesus macaque were euthanized by day 14. Serum alanine transaminase (ALT) and aspartate transaminase (AST) concentrations were elevated during the course of disease in all three species; however, AGMs had significantly higher ALT and AST concentrations than cynos and rhesus. While all three species had detectable viral load by days 3-4 post exposure, Rhesus had lower average peak viral load than cynos or AGMs. Overall, the results indicate that the disease course after exposure to aerosolized SUDV is similar for all three species of NHP. |
| Posted: 15 Oct 2012 12:00 AM PDT The blood-brain barrier is a substantial obstacle for delivering anticancer agents to brain tumors, and new strategies for bypassing it are sorely needed for brain tumor therapy. Intranasal delivery provides a practical, noninvasive method for delivering therapeutic agents to the brain. Intranasal application of nano-sized micelles that have been modified with Tat peptide facilitates brain delivery of fluorescent model materials. In this study, we evaluated a nose-to-brain delivery system for brain tumor therapy. We nasally administered the anti-tumor drug camptothecin (CPT) in solution and in methoxy poly(ethylene glycol) (MPEG)/poly(e-caprolactone) (PCL) amphiphilic block copolymers (MPEG-PCL) and cell penetrating peptide, Tat analog-modified MPEG-PCL (MPEG-PCL-Tat) MPEG-PCL-Tat to rats bearing intracranial glioma tumors and quantified the cytotoxicity against glioma cells, and the therapeutic effects. CPT-loaded MPEG-PCL-Tat micelles showed higher cytotoxicity than CPT-loaded MPEG-PCL. CPT-free MPEG-PCL-Tat didn't show any cytotoxicity, even at high concentrations (2 mmol/mL). CPT-loaded MPEG-PCL-Tat micelles significantly prolonged the median survival of rats. These results indicate that intranasal delivery of anti-cancer drugs with cell penetrating peptide-modified nanomicelles might be an effective therapy for brain tumors. |
| Posted: 15 Oct 2012 12:00 AM PDT The aim of this study was to investigate the association of breastfeeding duration and food variety at 2 years of age. A secondary data analysis was undertaken of the Western Australian Pregnancy Cohort (Raine) Study, an ongoing longitudinal study. Data collected from a single 24 h dietary recall of 1905, 2 year-old children were used to calculate two food variety scores; a core food variety score (CFVS) and a fruit and vegetable variety score (FVVS). Multivariate linear regression analysis was used to identify those factors independently associated with the CFVS and FVVS. The mean CFVS was 7.52 (range 1–18) of a possible 34 food items or groups and the mean FVVS was 2.84 (range 0–10) of a possible 16 food items or groups. Breastfeeding duration was independently directly associated with the CFVS (p < 0.001) and FVVS (p < 0.001). In addition, maternal age was independently directly associated with the CFVS (p < 0.001) and FVVS (p = 0.001) as was maternal education (CFVS p < 0.001 and FVVS p = 0.043). The presence of older siblings was independently inversely associated with the CFVS (p = 0.003) and FVVS (p = 0.001). This study demonstrated a direct modest association between breastfeeding duration and food variety in 2 year-old children, independent of maternal demographic characteristics known to predict food variety in children. This finding supports the hypothesis that flavours transferred in breast milk provide repeated early exposure to different tastes and positively shape children’s food preferences and food variety. |
| Posted: 15 Oct 2012 12:00 AM PDT Fifteen multi-national mining companies are currently members of the Earth Moving Equipment Safety Round Table (EMESRT). Formed in 2006, EMESRT engages with mining equipment manufacturers with the aim of accelerating improvements in the safe design of mining equipment. An initial stage in this process was to communicate industry understanding of the risks in the form of “design philosophies” that describe potential unwanted events in eight hazard categories. A technique for analysing risks associated with operation and maintenance tasks, the Operability and Maintainability Analysis Technique (OMAT), was subsequently developed and trialed. The next step is the EMESRT Design Evaluation for Equipment Procurement (EDEEP) process. The aims of requesting manufacturers to follow this process are to provide equipment purchasers with a common way of assessing how well the issues in the EMESRT Design Philosophies are addressed in the equipment design; and to provide manufacturers with additional information for use during equipment design. The process involves identifying priority tasks based on frequency and severity of the consequences of potential unwanted events identified in the EMESRT Design Philosophies; undertaking a task-based risk assessment of priority tasks in conjunction with site-based personnel; evaluating the effectiveness of control measures; and providing information about safe design features in a standardised format. |
| Golden Joystick Awards 2012: Best Browser/Flash category – who are you voting for? Posted: 15 Oct 2012 02:10 AM PDT As voting for the Golden Joystick Awards 2012 enters its final weeks, we take a look at each of the individual categories. Today is the turn of Best |
| Puzzler World Hits DSi And 3DS Posted: 15 Oct 2012 01:09 AM PDT UFO Interactive Games, Inc., a leading publisher of interactive entertainment, today announced that Puzzler World XL for the Nintendo DSi and Nintendo 3DS, is scheduled for release October 18 on the Nintendo's' DSiWare service. This posting includes an audio/video/photo media file: Download Now |
| Posted: 15 Oct 2012 12:00 AM PDT We present a scale-bridging approach for modeling the integral elasticresponse of polycrystalline composite that is based on a multi-disciplinary combination of(i) parameter-free first-principles calculations of thermodynamic phase stability andsingle-crystal elastic stiffness; and (ii) homogenization schemes developed forpolycrystalline aggregates and composites. The modeling is used as a theory-guidedbottom-up materials design strategy and applied to Ti-Nb alloys as promising candidatesfor biomedical implant applications. The theoretical results (i) show an excellent agreementwith experimental data and (ii) reveal a decisive influence of the multi-phase character ofthe polycrystalline composites on their integral elastic properties. The study shows thatthe results based on the density functional theory calculations at the atomistic level canbe directly used for predictions at the macroscopic scale, effectively scale-jumping severalorders of magnitude without using any empirical parameters. |
| Posted: 15 Oct 2012 12:00 AM PDT After finishing a human genome reference sequence in 2002, the genomics community has turned to the task of interpreting it. A primary focus is to identify and characterize not only protein-coding genes, but all functional elements in the genome. The effort includes both individual investigators and large-scale projects like the Encyclopedia of DNA Elements (ENCODE) project. As part of the ENCODE project, several groups have identified millions of regulatory elements in hundreds of human cell-types using DNase-seq and FAIRE-seq experiments that detect regions of nucleosome-free open chromatin. ChIP-seq experiments have also been used to discover transcription factor binding sites and map histone modifications. Nearly all identified elements are found in non-coding DNA, hypothesizing a function for previously unannotated sequence. In this review, we provide an overview of the ENCODE effort to define regulatory elements, summarize the main results, and discuss implications of the millions of regulatory elements distributed throughout the genome. |
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