понедельник, 3 декабря 2012 г.

Games

Games

Black Ops II wins UK chart again, Wii U exclusives debut outside top 10

Posted: 03 Dec 2012 02:45 AM PST

Despite another drop in sales (down 35% on last week), Call of Duty: Black Ops II enjoys a third week at number one in the latest UK chart compiled by

TotalSpaces 1.0.5 - Brings grid-style Spaces to OS X 10.7 and 10.8.. (Shareware)

Posted: 03 Dec 2012 02:29 AM PST



TotalSpaces is a desktop manager that brings back grid Spaces to OSX and more!
  • Define your grid - and navigate left, right, up and down between your desktops
  • Choose your transitions - Cube, slide and other effects, or turn them off altogether to really speed up your mac
  • Manage your desktops and windows from the Overview Grid - a birds-eye view of your grid
  • Hotkeys, hot-corners, circulation options and more


Version 1.0.5:
  • FIXED: Activating expose in the overview grid with many windows present (> 20) on a single space caused excessive CPU and delay


OS X 10.7 or later, Intel

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Folder Icon Changer 3.0.8 - Create custom icons for your Mac.. (Shareware)

Posted: 03 Dec 2012 02:35 AM PST



FolderIconCreator is an app to customize the icons on your Mac. The program comes pre-bundled with hundreds of icons that you can use to create custom folder icons and custom file icons on your Mac. Not only are their hundreds of icons included with the program, but you can create a library using ANY .png images, and convert them to icons easily using this program. You can make custom, personalized icons simply by using an image or photograph that you have (or downloaded from the internet), and dragging it into your custom icon library!You can assign custom icons in batches, or one at a time using drag-and-drop.The Folder Icon Maker comes pre-bundled with hundreds of icons, and we frequently release updates with additional icons. All updates are free forever!

Version 3.0.8:
  • Folder Icon Creator has been updated to comply with Apple File-System Usage Requirements.


Mac OS X 10.6 or later, Intel

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Students asked to plan serial murders

Posted: 03 Dec 2012 01:41 AM PST

Students have been asked to think like serial killers in a bizarre assignment. Picture: Actor Michael C Hall in a scene from TV show 'Dexter'. A GEELONG school Year 9 assignment did more than ask students to map out a serial killer's "dream house"; it also set tasks involving drawing cartoons of murders and designing psycho killer board games, it ... (more)

IJMS, Vol. 13, Pages 16418-16429: Temporal and Spatial Regulation of Ezrin-Radixin-Moesin-Binding Phosphoprotein-50-kDa (EBP50) during Embryo Implantation in Mouse Uterus

Posted: 03 Dec 2012 12:00 AM PST

Embryo implantation is a crucial process for successful pregnancy. To date, the mechanism of embryo implantation remains unclear. Ezrin-radixin-moesin-binding protein-50-kDa (EBP50) is a scaffold protein, which has been shown to play an important role in cancer development. Embryo implantation and cancer follow a similar progression. Thus, in this article, we utilized immunohistochemical staining and western blot analyses to examine the spatiotemporal expression and regulation of EBP50 both in the mouse uterus during embryo implantation as well as in other related models. We found that EBP50 was detected in epithelial cells in all of the groups used in our study. During the peri-implantation period, EBP50 mainly localized in apical membranes. At the implantation site (IS) on day 5 (D5) of pregnancy, EBP50 was mainly expressed in the nuclei of stroma cells, whereas from day 6 to day 8 (D6–D8) of pregnancy, the expression of EBP50 was noted in the cytoplasm of decidual cells. The expression of EBP50 was not significantly different in the pseudopregnant uterus and decreased in the uteri subjected to activation of delayed implantation. Artificial decidualization also decreased EBP50 expression. Thus, the expression levels and location were affected by active blastocysts and decidualization during the window of implantation.

IJMS, Vol. 13, Pages 16400-16417: Characterization of in Vitro Modified Human Very Low-Density Lipoprotein Particles and Phospholipids by Capillary Electrophoresis

Posted: 03 Dec 2012 12:00 AM PST

A simple capillary zone electrophoresis (CZE) method was used to characterize human very low-density lipoprotein (VLDL) particles for four healthy donors. One major peak was observed for native, in vitro oxidized and glycated VLDL particles. The effective mobilities and peak areas of the capillary electrophoresis (CE) profiles showed good reproducibility and precision. The mobility of the oxidized VLDL peak was higher than that of the native VLDL. The mobility of the glycated VLDL peak was similar to that of the native VLDL. Phospholipids isolated from VLDL particles were analyzed by our recently developed micellar electrokinetic chromatography (MEKC) with a high-salt stacking method. At absorbance 200 nm, the native VLDL phospholipids showed a major peak and a minor peak for each donor. For oxidized VLDL phospholipids, the area of the major peak reduced for three donors, possibly due to phospholipid decomposition. For glycated VLDL phospholipids, the peak mobilities were more positive than native VLDL phospholipids for two donors, possibly due to phospholipid-linked advanced glycation end products (AGEs). Very interestingly, at absorbance 234 nm, the major peak of oxidized VLDL phospholipids was resolved as two peaks for each donor, possibly due to conjugated dienes formed upon oxidation.

IJMS, Vol. 13, Pages 16387-16399: Photodegradable Polyesters for Triggered Release

Posted: 03 Dec 2012 12:00 AM PST

Photodegradable polyesters were synthesized with a photolabile monomer 2-nitrophenylethylene glycol and dioyl chlorides with different lengths. These polymers can be assembled to form polymeric particles with encapsulation of target substances. Light activation can degrade these particles and release payloads in both aqueous solutions and RAW 264.7 cells.

IJMS, Vol. 13, Pages 16373-16386: Integration of DNA Damage and Repair with Murine Double-Minute 2 (Mdm2) in Tumorigenesis

Posted: 03 Dec 2012 12:00 AM PST

The alteration of tumorigenic pathways leading to cancer is a degenerative disease process typically involving inactivation of tumor suppressor proteins and hyperactivation of oncogenes. One such oncogenic protein product is the murine double-minute 2, or Mdm2. While, Mdm2 has been primarily associated as the negative regulator of the p53 tumor suppressor protein there are many p53-independent roles demonstrated for this oncogene. DNA damage and chemotherapeutic agents are known to activate Mdm2 and DNA repair pathways. There are five primary DNA repair pathways involved in the maintenance of genomic integrity: Nucleotide excision repair (NER), Base excision repair (BER), Mismatch repair (MMR), Non-homologous end joining (NHEJ) and homologous recombination (HR). In this review, we will briefly describe these pathways and also delineate the functional interaction of Mdm2 with multiple DNA repair proteins. We will illustrate the importance of these interactions with Mdm2 and discuss how this is important for tumor progression, cellular proliferation in cancer.

IJMS, Vol. 13, Pages 16346-16372: Oxidative Stress in Malaria

Posted: 03 Dec 2012 12:00 AM PST

Malaria is a significant public health problem in more than 100 countries and causes an estimated 200 million new infections every year. Despite the significant effort to eradicate this dangerous disease, lack of complete knowledge of its physiopathology compromises the success in this enterprise. In this paper we review oxidative stress mechanisms involved in the disease and discuss the potential benefits of antioxidant supplementation as an adjuvant antimalarial strategy.

IJMS, Vol. 13, Pages 16333-16345: Large-Scale Isolation of Microsatellites from Chinese Mitten Crab Eriocheir sinensis via a Solexa Genomic Survey

Posted: 03 Dec 2012 12:00 AM PST

Microsatellites are simple sequence repeats with a high degree of polymorphism in the genome; they are used as DNA markers in many molecular genetic studies. Using traditional methods such as the magnetic beads enrichment method, only a few microsatellite markers have been isolated from the Chinese mitten crab Eriocheir sinensis, as the crab genome sequence information is unavailable. Here, we have identified a large number of microsatellites from the Chinese mitten crab by taking advantage of Solexa genomic surveying. A total of 141,737 SSR (simple sequence repeats) motifs were identified via analysis of 883 Mb of the crab genomic DNA information, including mono-, di-, tri-, tetra-, penta- and hexa-nucleotide repeat motifs. The number of di-nucleotide repeat motifs was 82,979, making this the most abundant type of repeat motif (58.54%); the second most abundant were the tri-nucleotide repeats (42,657, 30.11%). Among di-nucleotide repeats, the most frequent repeats were AC motifs, accounting for 67.55% of the total number. AGG motifs were the most frequent (59.32%) of the tri-nucleotide motifs. A total of 15,125 microsatellite loci had a flanking sequence suitable for setting the primer of a polymerase chain reaction (PCR). To verify the identified SSRs, a subset of 100 primer pairs was randomly selected for PCR. Eighty two primer sets (82%) produced strong PCR products matching expected sizes, and 78% were polymorphic. In an analysis of 30 wild individuals from the Yangtze River with 20 primer sets, the number of alleles per locus ranged from 2–14 and the mean allelic richness was 7.4. No linkage disequilibrium was found between any pair of loci, indicating that the markers were independent. The Hardy-Weinberg equilibrium test showed significant deviation in four of the 20 microsatellite loci after sequential Bonferroni corrections. This method is cost- and time-effective in comparison to traditional approaches for the isolation of microsatellites.

IJMS, Vol. 13, Pages 16303-16332: Sensing and Responding to UV-A in Cyanobacteria

Posted: 03 Dec 2012 12:00 AM PST

Ultraviolet (UV) radiation can cause stresses or act as a photoregulatory signal depending on its wavelengths and fluence rates. Although the most harmful effects of UV on living cells are generally attributed to UV-B radiation, UV-A radiation can also affect many aspects of cellular processes. In cyanobacteria, most studies have concentrated on the damaging effect of UV and defense mechanisms to withstand UV stress. However, little is known about the activation mechanism of signaling components or their pathways which are implicated in the process following UV irradiation. Motile cyanobacteria use a very precise negative phototaxis signaling system to move away from high levels of solar radiation, which is an effective escape mechanism to avoid the detrimental effects of UV radiation. Recently, two different UV-A-induced signaling systems for regulating cyanobacterial phototaxis were characterized at the photophysiological and molecular levels. Here, we review the current understanding of the UV-A mediated signaling pathways in the context of the UV-A perception mechanism, early signaling components, and negative phototactic responses. In addition, increasing evidences supporting a role of pterins in response to UV radiation are discussed. We outline the effect of UV-induced cell damage, associated signaling molecules, and programmed cell death under UV-mediated oxidative stress.

Now Playing - Strike Suit Zero

Posted: 03 Dec 2012 01:07 AM PST

Guy sits down with Born Ready Games' Jamin Smith for an exclusive first look at Strike Suit Zero.

 

Read and Post Comments | Get the full article at GameSpot

"Now Playing - Strike Suit Zero" was posted by sarahl on Mon, 03 Dec 2012 01:07:13 -0800

Now Playing: NBA2K13 Wii U

Posted: 02 Dec 2012 10:06 PM PST

Dan Chiappini sits down with producer Rob Jones to talk about the new features of NBA 2K13 for Wii U, as well as the process of developing games for Nintendo's new console and what's next for the NBA2K series.

 

Read and Post Comments | Get the full article at GameSpot

"Now Playing: NBA2K13 Wii U" was posted by edmondt on Sun, 02 Dec 2012 22:06:53 -0800

NaNiwa Talks future plans, Zerg balance,& more.

Posted: 02 Dec 2012 10:00 PM PST

Slasher chats with NaNiwa about his experience at IPL 5 and much more.

 

Read and Post Comments | Get the full article at GameSpot

"NaNiwa Talks future plans, Zerg balance,& more." was posted by andyb on Sun, 02 Dec 2012 22:00:28 -0800

IJMS, Vol. 13, Pages 16291-16302: In Vitro Antifungal Activity of Burkholderia gladioli pv. agaricicola against Some Phytopathogenic Fungi

Posted: 03 Dec 2012 12:00 AM PST

The trend to search novel microbial natural biocides has recently been increasing in order to avoid the environmental pollution from use of synthetic pesticides. Among these novel natural biocides are the bioactive secondary metabolites of Burkholderia gladioli pv. agaricicola (Bga). The aim of this study is to determine antifungal activity of Bga strains against some phytopathogenic fungi. The fungicidal tests were carried out using cultures and cell-free culture filtrates against Botrytis cinerea, Aspergillus flavus, Aspergillus niger, Penicillium digitatum, Penicillium expansum, Sclerotinia sclerotiorum and Phytophthora cactorum. Results demonstrated that all tested strains exert antifungal activity against all studied fungi by producing diffusible metabolites which are correlated with their ability to produce extracellular hydrolytic enzymes. All strains significantly reduced the growth of studied fungi and the bacterial cells were more bioactive than bacterial filtrates. All tested Bulkholderia strains produced volatile organic compounds (VOCs), which inhibited the fungal growth and reduced the growth rate of Fusarium oxysporum and Rhizoctonia solani. GC/MS analysis of VOCs emitted by strain Bga 11096 indicated the presence of a compound that was identified as 1-methyl-4-(1-methylethenyl)-cyclohexene, a liquid hydrocarbon classified as cyclic terpene. This compound could be responsible for the antifungal activity, which is also in agreement with the work of other authors.

David Ting Talks IPL's Future Plans & More

Posted: 02 Dec 2012 08:16 PM PST

General manager of IPL, David Ting talks to Slasher about what makes IPL strong and plans for continued success.

 

Read and Post Comments | Get the full article at GameSpot

"David Ting Talks IPL's Future Plans & More" was posted by andyb on Sun, 02 Dec 2012 20:16:25 -0800

IJMS, Vol. 13, Pages 16281-16290: Activation Energy of Aggregation-Disaggregation Self-Oscillation of Polymer Chain

Posted: 03 Dec 2012 12:00 AM PST

In this paper, we investigated the activation energies of the aggregation–disaggregation self-oscillation induced by the Belousov-Zhabotinsky (BZ) reaction by utilizing the nonthermoresponsive polymer chain in a wide temperature range. This is because the conventional type self-oscillating polymer chain, with thermoresponsive poly(Nisopropylacrylamide) (poly(NIPAAm) main-chain covalently bonded to the ruthenium catalyst (Ru(bpy)3) of the BZ reaction, cannot evaluate the activation energy over the lower critical solution temperature (LCST). The nonthermoresponsive self-oscillating polymer chain is composed of a poly-vinylpyrrolidone (PVP) main-chain with the ruthenium catalyst (Ru(bpy)3). As a result, we clarified that the activation energy of the aggregation–disaggregation self-oscillation of the polymer chain is hardly affected by the concentrations of the BZ substrates. In addition, the activation energy of the nonthermoresponsive self-oscillating polymer chain was found to be almost the same value as normal BZ reaction, i.e., not including the self-oscillating polymer system with Ru moiety.

IJMS, Vol. 13, Pages 16267-16280: Lysophosphatidylcholine Acyltransferase 3 Is the Key Enzyme for Incorporating Arachidonic Acid into Glycerophospholipids during Adipocyte Differentiation

Posted: 03 Dec 2012 12:00 AM PST

Cellular membranes contain glycerophospholipids, which have important structural and functional roles in cells. Glycerophospholipids are first formed in the de novo pathway (Kennedy pathway) and are matured in the remodeling pathway (Lands’ cycle). Recently, lysophospholipid acyltransferases functioning in Lands’ cycle were identified and characterized. Several enzymes involved in glycerophospholipid biosynthesis have been reported to have important roles in adipocytes. However, the role of Lands’ cycle in adipogenesis has not yet been reported. Using C3H10T1/2, a cell line capable of differentiating to adipocyte-like cells in vitro, changes of lysophospholipid acyltransferase activities were investigated. Lysophosphatidylcholine acyltransferase (LPCAT), lysophosphatidylethanolamine acyltransferase (LPEAT) and lysophosphatidylserine acyltransferase (LPSAT) activities were enhanced, especially with 18:2-CoA and 20:4-CoA as donors. Correspondingly, mRNA expression of LPCAT3, which possesses LPCAT, LPEAT and LPSAT activities with high specificity for 18:2- and 20:4-CoA, was upregulated during adipogenesis. Analysis of acyl-chain compositions of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) showed a change in their profiles between preadipocytes and adipocytes, including an increase in the percentage of arachidonic acid-containing phospholipids. These changes are consistent with the activities of LPCAT3. Therefore, it is possible that enhanced phospholipid remodeling by LPCAT3 may be associated with adipocyte differentiation.

Sensors, Vol. 12, Pages 16614-16627: Development of a Novel, Fully-Automated Genotyping System: Principle and Applications

Posted: 03 Dec 2012 12:00 AM PST

Genetic testing prior to treatment, pharmacogenetic analysis, is key to realizing personalized medicine which is a topic that has attracted much attention recently. Through the optimization of therapy selection and dosage, a reduction in side effects is expected. Genetic testing has been conducted as a type of pharmacogenetic analysis in recent years, but it faces challenges in terms of cost effectiveness and its complicated procedures. Here we report on the development of a novel platform for genetic testing, the i-densyTM, with the use of quenching probe system (QP-system) as principle of mutant detection. The i-densyTM automatically performs pre-treatment, PCR and detection to provide the test result from whole blood and extracted DNA within approximately 90 and 60 min, respectively. Integration of all steps into a single platform greatly reduces test time and complicated procedures. An even higher-precision genetic analysis has been achieved through the development of novel and highly-specific detection methods. The applications of items measured using the i-densyTM are diverse, from single nucleotide polymorphism (SNP), such as CYP2C19 and UGT1A1, to somatic mutations associated with cancer, such as EGFR, KRAS and JAK2. The i-densyTM is a useful tool for optimization of anticancer drug therapy and can contribute to personalized medicine.

Sensors, Vol. 12, Pages 16591-16613: Network Adaptive Deadband: NCS Data Flow Control for Shared Networks

Posted: 03 Dec 2012 12:00 AM PST

This paper proposes a new middleware solution called Network Adaptive Deadband (NAD) for long time operation of Networked Control Systems (NCS) through the Internet or any shared network based on IP technology. The proposed middleware takes into account the network status and the NCS status, to improve the global system performance and to share more effectively the network by several NCS and sensor/actuator data flows. Relationship between network status and NCS status is solved with a TCP-friendly transport flow control protocol and the deadband concept, relating deadband value and transmission throughput. This creates a deadband-based flow control solution. Simulation and experiments in shared networks show that the implemented network adaptive deadband has better performance than an optimal constant deadband solution in the same circumstances.
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