среда, 12 октября 2011 г.

Games

Games

Monsieur Monsieur - Videos and Trailers

Posted: 12 Oct 2011 03:28 AM PDT

Monsieur Monsieur trailer.

The Adventures of Timmy: Run, Kitty, Run - Videos and Trailers

Posted: 12 Oct 2011 03:25 AM PDT

The Adventures of Timmy: Run, Kitty, Run trailer.

IJMS, Vol. 12, Pages 6743-6748: A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae)

Posted: 12 Oct 2011 12:00 AM PDT

Luculia gratissima (Wall.) Sweet (Rubiaceae) is a perennial shrub distributed in the southeast margin of the Tibetan Plateau in southwest China and adjacent region of Nepal and Myanmar. The plant is a distylous species with reciprocally placed stigmas and anthers in each floral morph. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing (FIASCO) repeats protocol, 19 primer sets were identified in two wild populations. Of these primers, 10 displayed polymorphisms and nine were monomorphic. The number of alleles per locus ranged from two to five, values for observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.289 to 0.760, with averages of 0.303 and 0.555, respectively. These microsatellite loci will facilitate further studies on breeding system, gene flow patterns, and population structure of L. gratissima and its allied species.

Pharmaceutics, Vol. 3, Pages 680-705: Transporter-Mediated Drug–Drug Interactions with Oral Antidiabetic Drugs

Posted: 12 Oct 2011 12:00 AM PDT

Uptake transporters (e.g., members of the SLC superfamily of solute carriers) and export proteins (e.g., members of the ABC transporter superfamily) are important determinants for the pharmacokinetics of drugs. Alterations of drug transport due to concomitantly administered drugs that interfere with drug transport may alter the kinetics of drug substrates. In vitro and in vivo studies indicate that many drugs used for the treatment of metabolic disorders and cardiovascular diseases (e.g., oral antidiabetic drugs, statins) are substrates for uptake transporters and export proteins expressed in the intestine, the liver and the kidney. Since most patients with type 2 diabetes receive more than one drug, transporter-mediated drug-drug interactions are important molecular mechanisms leading to alterations in oral antidiabetic drug pharmacokinetics with the risk of adverse drug reactions. This review focuses on uptake transporters of the SLCO/SLC21 (OATP) and SLC22 (OCT/OAT) family of solute carriers and export pumps of the ABC (ATP-binding cassette) transporter superfamily (especially P-glycoprotein) as well as the export proteins of the SLC47 (MATE) family and their role for transporter-mediated drug-drug interactions with oral antidiabetic drugs.

Polymers, Vol. 3, Pages 1776-1793: Regeneration Approaches for Dental Pulp and Periapical Tissues with Growth Factors, Biomaterials, and Laser Irradiation

Posted: 12 Oct 2011 12:00 AM PDT

In current dental practice, restorative and endodontic procedures have been developed in an attempt to preserve the vitality of dental pulp after exposure to external stimuli such as caries infection. When damage to dental pulp is reversible, pulp wound healing can proceed, whereas irreversible damage induces pathological changes in dental pulp, eventually requiring its removal. Furthermore, dentists sometimes extract non-vital teeth because of severe caries progression, critical size of periapical lesion, and tooth fracture. To overcome the limitations of presently available therapies, it is important to develop regeneration therapy for dental pulp and periapical tissues. In this review, we focus on the regeneration of dental pulp and periapical tissues by application of exogenous growth factors and scaffolds, as well as low-intensity laser irradiation as an auxiliary therapy for regeneration therapy.

Merlin 2.8.1 - Project management software.. (Demo)

Posted: 12 Oct 2011 03:11 AM PDT



Merlin is the only native network-based collaborative Project Management solution for Mac OS X.

This version offers many features propelling Merlin to the top of Mac OS X professional project management software.

Here are some of the main highlights in Merlin 2:

  • Merlin has now a web module which is included in this version of Merlin. The following browsers are supported: Safari 3, Firefox 2.0+ and Internet Explorer 7.0
  • Merlin now supports special Mac OS X 10.7 features like full screen and restoring last edited files.
  • Network-based Collaborative Project Management
    Collaborate with others on the same project file over the LAN or WAN with a single mouse click.
  • Access Control for Users and User Groups
    Resources can be either single Users or grouped into Resource Groups both of which can be assigned to activities and/or given specific access rights.
  • Dynamic Planning Mode
    Activities which haven't started yet can be automatically moved to the current date. This is great news for devotees of Agile Project Management.
  • Bi-directional Synchronization with iCal
    Changes in Merlin are reflected in iCal and vice versa. You can also sync specific resources in a separate calendar.
  • Enhanced Financial and Cost Models
    You can now compare planned vs. actual costs with the ability to determine accruals per cost entry.



Version 2.8.1:
  • NovaMind 5 files can be imported now.
  • In Merlin lost licenses can be requested now.

Resolved Issues in Merlin:

  • MindManager files exported from Merlin could not be opend by MindManager 9 under Windows.
  • Importing of CSV files could mess up when some key fields were empty.
  • Completed milestones from OmniPlan 1 were not completed after import in Merlin.
  • The import of contacts from LDAP servers did not work correctly.
Resolved Issues in Merlin Web:
  • Projects containing an UniCode linebreak in texfields could not be displayed in the the web.
  • Printing did not work properly.


Mac OS X 10.4.11 or later

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Acorn 3.1.2 - Bitmap image editor.. (Demo)

Posted: 12 Oct 2011 02:48 AM PDT



Acorn is a new image editor built with one goal in mind - simplicity. Fast, easy, and fluid, Acorn provides the options you'll need without any overhead. Acorn feels right, and won't drain your bank account.
  • Take screenshots using Acorn and edit them right away.
  • Chain together image filters to create stunning effects.
  • Layer based image editing, an industry standard.
  • Make new images and layers using your built-in iSight.
  • Easy image and canvas resizing, just by changing the size of your window.
  • Take advantage of every pixel of your monitor with full screen image editing.
  • Tablet sensitive for pressure strokes and using the tablet's eraser.
  • Vector shape and text layers.
  • Freeform, elliptical, rectangular, and magic wand selections.
  • Gradients.
  • Create and apply custom text styles.
  • Control opacity and blending modes for each layer.
  • Write plugins using the Python scripting language, as well as in Objective-C.
  • GPU powered. The same graphics card that makes your gaming experience smooth, helps Acorn fly through the toughest of graphics operations.


Version 3.1.2:
  • Fixed a possible crasher when using the gradient tool.
  • Change: To scale the image while resizing the window, you now have to hold down both the command and option keys (previously it only took the option key). This change was made because on 10.7, holding down the option key when resizing a window from a corner will move all corners at the same time.
  • Fix: Acorn would draw slowly when working on a shape layer and you had a New View window open at the same time.
  • Fix: An issue where a Acorn would crash when opening up the iPhoto Library has been corrected.
  • Fix: An issue where holding down the option key with the brush tool selected would not update the brush preview correctly with a new color.
  • Fix: An issue where a bitmap layer could disapear when changing the color profile has been fixed.
  • Fixed some memory leaks.


Mac OS X 10.6.6 or later

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Pioneer One: crowdfunded science fiction TV series

Posted: 12 Oct 2011 03:05 AM PDT

Jamie writes in about Pioneer One, "A drama series with a sci-fi bent, made independently and distributed online. It gained some notoriety for being the first 'TV show' to be distributed through BitTorrent via VODO.net and has thus far been funded entirely by donations from its viewers. If you're looking for breakneck action and glitzy Hollywood polish, you probably won't be into this show. This is truly 'guerrilla television,' kind of a new phenomenon made possible by the web. But there is a story being told here that will reward a viewer who invests in it. 5 full-length episodes have been released, with the finale of the first season to come by December."

Yasumi Matsuno and Level-5 team up for Guild 01

Posted: 12 Oct 2011 02:47 AM PDT

Four games contained inside 3DS title; Suda 51 and Yoot Saito contributing for project.

A few months ago, game publisher Level-5 mentioned that renowned developer Yasumi Matsuno had joined the company and would be making a new game. Recent news on the latest issue of Japanese magazine Famitsu (via Andriasang) revealed that this new game will be called Guild 01 for the Nintendo 3DS. Furthermore, the game will also have a few other well-known developers on board: Grasshopper Manufacture's Goichi "Suda 51" Suda and Vivarium's Yoot Saito (Seaman, The Tower).

Guild 01 will include four different games of different genres individually created by the aforementioned developers. The first game is called Rental Bukiya de Omasse, an RPG developed by Yoshiyuki Hirai, who is a member of the Japanese comedy group America Zarigani. Gamers take control of a weapon shop owner who has to rent out and sell crafted armaments to RPG adventurers. Players will also have to make use of the "donai natter", a communication device that tracks the exploits of the adventurers from afar and the items they rent from the player's store.

The next game is a 3D shooter called Kaiho Shojo, where players control a "liberation machine" robot--piloted by a female president of "New Japan" called President Shoko--tasked with destroying demons and beasts. Gamers will need to control the robot using touch screen controls. This part of the game is developed by Suda 51 and will feature animation from anime studio Bones.

Yoot Saito will be developing a game called Air Porter which is billed as an "airport luggage management simulator". Here, gamers control an airport manager who has to handle other people's baggage that pass through security and into the designated flights. Players earn points by getting the luggage into the planes on time. Successfully managing bag transport will expand the size of the airport.

Finally, Matsuno will be developing an RPG called Crimson Shroud. Players control a chaser, which is a type of person who is skilled at tracking people. The game's story takes place in a dark fantasy setting where magic does not exist. Former Square Enix artist Hideo Minaba will be working alongside Matsuno in creating the game's artwork.

The game will be out in Japan in 2012. An English version of the game has yet to be confirmed.

Read and Post Comments | Get the full article at GameSpot

"Yasumi Matsuno and Level-5 team up for Guild 01" was posted by Jonathan Leo Toyad on Wed, 12 Oct 2011 02:47:18 -0700

Sony locks down PSN, SOE accounts after security breach

Posted: 11 Oct 2011 10:49 PM PDT

Sony locks down 93,000 PSN, SOE, and SEN user accounts after detecting a large number of unauthorised attempts to gain access.

In April this year, Sony's PlayStation Network was subject to a widespread security breach, which led to a month-long network outage and the exposure of some 77 million PSN accounts and an additional 24.6 million Sony Online Entertainment accounts.

Although Sony managed to restore all affected PSN services by June, it looks like the publisher may once again be the subject of yet another attack.

In a statement to media today, and as reported byCNET, Sony revealed that it has locked down 93,000 user accounts on its PlayStation Network, Sony Entertainment Network, and Sony Online Entertainment network after detecting a large number of unauthorised attempts to gain access to the accounts.

According to Sony, intruders "using very large sets of sign-in IDs and passwords" had brief access to 60,000 accounts on the PlayStation Network and Sony Entertainment Network, and another 33,000 accounts on the Sony Online Entertainment network.

In a post on the official PlayStation blog, Sony chief information security officer Philip Reitinger said that the attacks have affected less than one tenth of 1 percent of customers.

"As a preventative measure, we are requiring secure password resets for those PSN/SEN accounts that had both a sign-in ID and password match through this attempt," Reitinger said in the post. "If you are in the small group of PSN/SEN users who may have been affected, you will receive an email from us at the address associated with your account that will prompt you to reset your password."

According to Reitinger, hackers succeeded in verifying sign-in IDs and passwords, but credit card information was not breached. Sony locked the accounts after confirming that the attempts were unauthorised, and is sending emails to affected consumers who have locked accounts.

Read and Post Comments | Get the full article at GameSpot

"Sony locks down PSN, SOE accounts after security breach" was posted by Laura Parker on Tue, 11 Oct 2011 22:49:41 -0700

Sensors, Vol. 11, Pages 9685-9699: Design of Highly Sensitive C2H5OH Sensors Using Self-Assembled ZnO Nanostructures

Posted: 12 Oct 2011 12:00 AM PDT

Various ZnO nanostructures such as porous nanorods and two hierarchical structures consisting of porous nanosheets or crystalline nanorods were prepared by the reaction of mixtures of oleic-acid-dissolved ethanol solutions and aqueous dissolved Zn-precursor solutions in the presence of NaOH. All three ZnO nanostructures showed sensitive and selective detection of C2H5OH. In particular, ultra-high responses (Ra/Rg = ~1,200, Ra: resistance in air, Rg: resistance in gas) to 100 ppm C2H5OH was attained using porous nanorods and hierarchical structures assembled from porous nanosheets, which is one of the highest values reported in the literature. The gas response and linearity of gas sensors were discussed in relation to the size, surface area, and porosity of the nanostructures.

Sensors, Vol. 11, Pages 9667-9684: Terbium to Quantum Dot FRET Bioconjugates for Clinical Diagnostics: Influence of Human Plasma on Optical and Assembly Properties

Posted: 12 Oct 2011 12:00 AM PDT

Förster resonance energy transfer (FRET) from luminescent terbium complexes (LTC) as donors to semiconductor quantum dots (QDs) as acceptors allows extraordinary large FRET efficiencies due to the long Förster distances afforded. Moreover, time-gated detection permits an efficient suppression of autofluorescent background leading to sub-picomolar detection limits even within multiplexed detection formats. These characteristics make FRET-systems with LTC and QDs excellent candidates for clinical diagnostics. So far, such proofs of principle for highly sensitive multiplexed biosensing have only been performed under optimized buffer conditions and interactions between real-life clinical media such as human serum or plasma and LTC-QD-FRET-systems have not yet been taken into account. Here we present an extensive spectroscopic analysis of absorption, excitation and emission spectra along with the luminescence decay times of both the single components as well as the assembled FRET-systems in TRIS-buffer, TRIS-buffer with 2% bovine serum albumin, and fresh human plasma. Moreover, we evaluated homogeneous LTC-QD FRET assays in QD conjugates assembled with either the well-known, specific biotin-streptavidin biological interaction or, alternatively, the metal-affinity coordination of histidine to zinc. In the case of conjugates assembled with biotin-streptavidin no significant interference with the optical and binding properties occurs whereas the histidine-zinc system appears to be affected by human plasma.

Sensors, Vol. 11, Pages 9658-9666: Numerical Optimization of a Microfluidic Assisted Microarray for the Detection of Biochemical Interactions

Posted: 12 Oct 2011 12:00 AM PDT

Finite element method analysis was applied to the characterization of the biomolecular interactions taking place in a microfluidic assisted microarray. Numerical simulations have been used for the optimization of geometrical and physical parameters of the sensing device. Different configurations have been analyzed and general considerations have been derived. We have shown that a parallel disposition of the sensing area allows the homogeneous formation of the target molecular complex in all the active zones of the microarray. Stationary and time dependent results have also been obtained.

Sensors, Vol. 11, Pages 9628-9657: Adaptive Road Crack Detection System by Pavement Classification

Posted: 12 Oct 2011 12:00 AM PDT

This paper presents a road distress detection system involving the phases needed to properly deal with fully automatic road distress assessment. A vehicle equipped with line scan cameras, laser illumination and acquisition HW-SW is used to storage the digital images that will be further processed to identify road cracks. Pre-processing is firstly carried out to both smooth the texture and enhance the linear features. Non-crack features detection is then applied to mask areas of the images with joints, sealed cracks and white painting, that usually generate false positive cracking. A seed-based approach is proposed to deal with road crack detection, combining Multiple Directional Non-Minimum Suppression (MDNMS) with a symmetry check. Seeds are linked by computing the paths with the lowest cost that meet the symmetry restrictions. The whole detection process involves the use of several parameters. A correct setting becomes essential to get optimal results without manual intervention. A fully automatic approach by means of a linear SVM-based classifier ensemble able to distinguish between up to 10 different types of pavement that appear in the Spanish roads is proposed. The optimal feature vector includes different texture-based features. The parameters are then tuned depending on the output provided by the classifier. Regarding non-crack features detection, results show that the introduction of such module reduces the impact of false positives due to non-crack features up to a factor of 2. In addition, the observed performance of the crack detection system is significantly boosted by adapting the parameters to the type of pavement.

PdfBoxEditor 1.0 - Edit, view, and inspect PDF boxes.. (Commercial)

Posted: 12 Oct 2011 02:40 AM PDT



PdfBoxEditor provides you a handy and powerful tool for editing, viewing and inspecting PDF boxes. Some typical tasks include:
  • Before you submit your project to a printing company, you should thoroughly check if all required boxes are present in your ready-to-print PDF and if they align correctly with the printer's marks.
  • When you receive PDF files for further processing, you have to get an overview of the boxes defined by other authors. In case of missing or misaligned boxes, you will have to correct the settings and copy them to all applicable pages.
  • Before you distribute your PDF documents, reassure that no confidential data is contained outside the visible area defined by the CropBox.
  • You have got PDF documents with printer's marks but without PDF boxes. Simply add the missing boxes - e.g. the TrimBox - and use the guides in the PDF preview to quickly and precisely align the box with the printer's marks.
  • Your PDF document has white margins which you would like to reduce for printing or display on a mobile device. Create a CropBox around the relevant page area and copy the box settings to all other pages.
Key Features:
  • PDF preview with highlighted box areas
  • guides to precisely check box alignment with printer's marks
  • selected boxes can be copied to all, even, or odd pages
  • redefinition of boxes based on graphical adjustments or textual inputs
  • creation of missing boxes
  • tabular overview of all box settings
  • available units: cm, inch, mm, pt
  • undo functions
Interface Languages:
  • English, German


Initial release

  • Mac OS X 10.6.6 or later.
  • 64-bit processor


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Flirt 1.0.10 - Manage your Facebook accounts, pages and groups.. (Demo)

Posted: 12 Oct 2011 02:34 AM PDT



Flirt is your secret weapon to marketing on Facebook. You need more likes. Maybe for your band, a product, event or group. You need to quickly invite people to your event. You manage several pages or groups and need to increase the number of fans.

Then you need Flirt. The #1 native Mac OS X professional Facebook desktop application. We've designed Flirt from scratch to bring you a truly new and effective solution that is turbo-charged, secure, and exceptional.

With Flirt you can effectively manage your Facebook account, pages, events and groups. It's easy to add friends or fans, invite them to your events, or send them an email. And it's a real deal, over $500 less than Facebook FriendAdder Pro!

Standard ($29.99) and Pro ($89.99) versions available.



Version 1.0.10:
  • This update fixes compatibility with recent Facebook changes to sending event invites and page suggestions.


Mac OS X 10.6.6 or later

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FountainPen 0.7.3 - Simple and clean writing environment.. (Free)

Posted: 12 Oct 2011 02:18 AM PDT



FountainPen is a simple and clean writing environment specifically designed for distraction-free composition. Featuring autosaved compositions, FountainPen also includes integrated support for the Markdown system of plain-text formatting. It can also put up a backdrop-a sort of second desktop picture-over visual clutter to aid focus.

Version 0.7.3:
  • Improved Compatibility with Mac OS X 10.7 Lion: on launch, FountainPen previously opened the compositions you had open last time you quit. FountainPen now trusts Mac OS X 10.7 (if present) to do this, which will alleviate stability issues at launchtime.
  • Enhanced Output Features Copy to Clipboard: in Revision 1 we added the ability to copy the text out of the Output preview panel, either as rich text or as raw HTML source. All previous features, of course, remain intact.

Please Note: We'd like to apologize if you receive an error while trying to update FountainPen. The problem should be fixed shortly. You can always download the latest version of FountainPen using the above link.



Mac OS X 10.6 or later.

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BusyCal 1.6 - Powerful calendar app with multiple syncing options.. (Shareware)

Posted: 12 Oct 2011 02:24 AM PDT



BusyCal is an award winning desktop calendar that combines personal productivity features for individuals with powerful calendar sharing capabilities for families and workgroups.

BusyCal's unique features include a non-modal Info Panel for easier data entry; To Dos that display in the calendar and auto-forward until completed; repeating To Dos; customizable calendar views including a List view and scrolling Month and Week views; adjustable font styles and sizes, live weather feeds, moon phases, graphics, sticky notes and more.

BusyCal allows families and workgroups to share calendars with MobileMe, Google Calendar, iCal Server, and other CalDAV Servers. You can even share calendars with other BusyCal users on a local area network without the need of a server. And BusyCal syncs with iCal, iPhone, iPad, iPod Touch, and other apps and devices that sync with MobileMe or Sync Services.

Features:

  • View and edit event details in a non-modal info panel.
  • Create repeating To Dos that display in the calendar and carry-forward until completed.
  • Scrolling Month and Week Views.
  • Customizable list view lets you filter and sort events by date, calendar, and more.
  • View live weather forecasts, sunrise/sunset times, and moon phases right in your calendar.
  • Customizable event fonts, sizes, styles and colors.
  • Add graphics to your calendar.
  • Add virtual sticky notes to your calendar.
  • Sync with the iPad, iPhone, and iPod Touch via MobileMe, Google Calendar, or iTunes.
  • Sync with MobileMe Calendar and other computers/devices that sync with MobileMe.
  • Sync with Google Calendar and other computers/devices that sync with Google Calendar.
  • Sync with other BusyCal users on a local area network or over the internet.
  • Share calendars with others, with password-protection and SSL encryption.

    • iCal compatible:

    When you first launch BusyCal, all of your iCal data is instantly imported. BusyCal automatically syncs with iCal, iPhone, iPad, iPod Touch, and other apps and devices that sync with MobileMe or Sync Services. If you later decide to switch back to iCal, all of your calendar data will be current.



    Version 1.6:

    What's new:

    • Added support for iCloud.
    • Added support for Location alarms when syncing with the Reminders app or BusyToDo on iOS 5.
    • Added support for syncing repeating To Dos with the Reminders app on iOS 5.
    • Added support for absolute alarms on undated tasks.
    • Added ability to move calendars to iCloud.
    • Added ability to accept iCloud shared calendar requests.
    • Added ability to change name and color of shared iCloud calendars independent of host settings.
    • Added ability to print multiple days/weeks/months.
    • Fixed Text Size and List View printing bugs.
    • Fixed full screen mode bugs on Mac OS X 10.7 Lion.
    • Fixed Mac OS X 10.7 Lion iCal Server syncing bug when completing undated To Dos.
    • Fixed bug in filename when performing a manual backup.
    • Fixed crash that could occur when dragging an email, contact, or image onto an event.
    • Fixed crash when modifying a custom alarm or repeat.
    • Fixed crash that could occur if initial sync was aborted.
    WARNING: If you plan to use iCloud, please be aware of the following:
    • iCloud requires Mac OS X 10.7 Lion.
    • iCloud calendars can be shared only with other iCloud users.
    • iCloud stores Events and To Dos on separate calendars.
    • iCloud handles repeating To Dos differently than MobileMe.
    BusyCal 1.6 includes support for iCloud and adds other enhancements and bug fixes. This is a free update that is recommended for all users, whether or not you use iCloud. To learn more, please read Upgrading BusyCal to iCloud, before you upgrade to iCloud.



    Mac OS X 10.5.7 or later.

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    Webbla 1.5.0.25 - Organize your bookmarks visually (Beta).. (Shareware)

    Posted: 12 Oct 2011 01:50 AM PDT



    Webbla allows you to organize your Bookmarks visually with Delicious and Pinboard support.

    If you like to handle your music and photos with iTunes and iPhoto, Webbla is the right application to handle your bookmarks.

    Webbla is a modern bookmark manager for Mac OS X Leopard that helps you in organizing your bookmarks visually. It also offers you an easy way to keep track of website updates. Instead of remembering your bookmarks by title, Webbla gives you a new way to remember them visually. Personalizing your bookmarks with tags or keeping them in different categories will help you to come back to them easily.

    Browser compatibility Define your own shortcuts to add bookmarks from your web browser to Webbla. Customizable web browser scripts make it easy to open bookmarks in different ways with all applications supporting AppleScript.

    Over standard html bookmark files you can import and export your bookmarks to and from Camino, Firefox, OmniWeb, Opera, Safari and more.

    Mac OS X System integration
    Webbla support Spotlight, Cover Flow and Quick Look, so you can easily find and preview your bookmarks with the Finder.



    Version 1.5.0.25
    • Preview catcher stability & performance.
    • Bookmark update fuctionality fix.
    • OuickLook and Spotlight fix.
    • Other Bug fixes and improvements.


    • Mac OS X 10.6.8 or later.
    • 64-bit processor


    Download Now

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    Sensors, Vol. 11, Pages 9613-9627: Cell Docking, Movement and Cell-Cell Interactions of Heterogeneous Cell Suspensions in a Cell Manipulation Microdevice

    Posted: 12 Oct 2011 12:00 AM PDT

    This study demonstrates a novel cell manipulation microdevice for cell docking, culturing, cell-cell contact and interaction by microfluidic manipulation of heterogeneous cell suspensions. Heterogeneous cell suspensions include disparate blood cells of natural killer cells and leukemia cancer cells for immune cell transplantation therapy. However, NK cell alloreactivity from different healthy donors present various recovery response levels. Little is still known about the interactions and cytotoxicity effects between donor NK cells and recipient cancer cells. The cell-based micro device first showed the capability of cell docking, movement, contact and cell-cell interaction with respect to cell cytotoxicity of NK cells against cancer cells. With various flow tests for live cell loading, flow rates of 10 μL/h were chosen for injection in the central and side flows such that both types of suspension cells could be gently docked at the gap structure in a reaction zone. The trapping number of particles and cells was linearly proportional to the gap length. Finally, the cytotoxicity of around 40% was found to be similar in the case of dilute cells and a large cell population. As a result, the cell manipulation microdevice has been validated for live suspensions of natural killer and cancer cells, and exhibited the capability to measure the cytotoxicity of dilute cell suspensions.
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