среда, 6 марта 2013 г.

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IJMS, Vol. 14, Pages 5402-5431: Mass Spectrometry-Based Proteomics for the Analysis of Chromatin Structure and Dynamics

Posted: 06 Mar 2013 12:00 AM PST

Chromatin is a highly structured nucleoprotein complex made of histone proteins and DNA that controls nearly all DNA-dependent processes. Chromatin plasticity is regulated by different associated proteins, post-translational modifications on histones (hPTMs) and DNA methylation, which act in a concerted manner to enforce a specific "chromatin landscape", with a regulatory effect on gene expression. Mass Spectrometry (MS) has emerged as a powerful analytical strategy to detect histone PTMs, revealing interplays between neighbouring PTMs and enabling screens for their readers in a comprehensive and quantitative fashion. Here we provide an overview of the recent achievements of state-of-the-art mass spectrometry-based proteomics for the detailed qualitative and quantitative characterization of histone post-translational modifications, histone variants, and global interactomes at specific chromatin regions. This synopsis emphasizes how the advances in high resolution MS, from "Bottom Up" to "Top Down" analysis, together with the uptake of quantitative proteomics methods by chromatin biologists, have made MS a well-established method in the epigenetics field, enabling the acquisition of original information, highly complementary to that offered by more conventional, antibody-based, assays.

IJMS, Vol. 14, Pages 5380-5401: Roles of Melatonin in Fetal Programming in Compromised Pregnancies

Posted: 06 Mar 2013 12:00 AM PST

Compromised pregnancies such as those associated with gestational diabetes mellitus, intrauterine growth retardation, preeclampsia, maternal undernutrition, and maternal stress may negatively affect fetal development. Such pregnancies may induce oxidative stress to the fetus and alter fetal development through the epigenetic process that may affect development at a later stage. Melatonin is an oxidant scavenger that reverses oxidative stress during the prenatal period. Moreover, the role of melatonin in epigenetic modifications in the field of developmental programming has been studied extensively. Here, we describe the physiological function of melatonin in pregnancy and discuss the roles of melatonin in fetal programming in compromised pregnancies, focusing on its involvement in redox and epigenetic mechanisms.

IJMS, Vol. 14, Pages 5367-5379: Endometriosis-Associated Ovarian Cancer: A Review of Pathogenesis

Posted: 06 Mar 2013 12:00 AM PST

Endometriosis is classically defined as the presence of endometrial glands and stroma outside of the endometrial lining and uterine musculature. With an estimated frequency of 5%–10% among women of reproductive age, endometriosis is a common gynecologic disorder. While in itself a benign lesion, endometriosis shares several characteristics with invasive cancer, has been shown to undergo malignant transformation, and has been associated with an increased risk of epithelial ovarian carcinoma (EOC). Numerous epidemiologic studies have shown an increased risk of EOC among women with endometriosis. This is particularly true for women with endometrioid and clear cell ovarian carcinoma. However, the carcinogenic pathways by which endometriosis associated ovarian carcinoma (EAOC) develops remain poorly understood. Current molecular studies have sought to link endometriosis with EAOC through pathways related to oxidative stress, inflammation and hyperestrogenism. In addition, numerous studies have sought to identify an intermediary lesion between endometriosis and EAOC that may allow for the identification of endometriosis at greatest risk for malignant transformation or for the prevention of malignant transformation of this common gynecologic disorder. The objective of the current article is to review the current data regarding the molecular events associated with EAOC development from endometriosis, with a primary focus on malignancies of the endometrioid and clear cell histologic sub-types.

IJMS, Vol. 14, Pages 5338-5366: Exosomes as Intercellular Signaling Organelles Involved in Health and Disease: Basic Science and Clinical Applications

Posted: 06 Mar 2013 12:00 AM PST

Cell to cell communication is essential for the coordination and proper organization of different cell types in multicellular systems. Cells exchange information through a multitude of mechanisms such as secreted growth factors and chemokines, small molecules (peptides, ions, bioactive lipids and nucleotides), cell-cell contact and the secretion of extracellular matrix components. Over the last few years, however, a considerable amount of experimental evidence has demonstrated the occurrence of a sophisticated method of cell communication based on the release of specialized membranous nano-sized vesicles termed exosomes. Exosome biogenesis involves the endosomal compartment, the multivesicular bodies (MVB), which contain internal vesicles packed with an extraordinary set of molecules including enzymes, cytokines, nucleic acids and different bioactive compounds. In response to stimuli, MVB fuse with the plasma membrane and vesicles are released in the extracellular space where they can interact with neighboring cells and directly induce a signaling pathway or affect the cellular phenotype through the transfer of new receptors or even genetic material. This review will focus on exosomes as intercellular signaling organelles involved in a number of physiological as well as pathological processes and their potential use in clinical diagnostics and therapeutics.

IJMS, Vol. 14, Pages 5312-5337: Cold Signaling and Cold Response in Plants

Posted: 06 Mar 2013 12:00 AM PST

Plants are constantly exposed to a variety of environmental stresses. Freezing or extremely low temperature constitutes a key factor influencing plant growth, development and crop productivity. Plants have evolved a mechanism to enhance tolerance to freezing during exposure to periods of low, but non-freezing temperatures. This phenomenon is called cold acclimation. During cold acclimation, plants develop several mechanisms to minimize potential damages caused by low temperature. Cold response is highly complex process that involves an array of physiological and biochemical modifications. Furthermore, alterations of the expression patterns of many genes, proteins and metabolites in response to cold stress have been reported. Recent studies demonstrate that post-transcriptional and post-translational regulations play a role in the regulation of cold signaling. In this review article, recent advances in cold stress signaling and tolerance are highlighted.

IJMS, Vol. 14, Pages 5284-5311: Inhibition of Human Transthyretin Aggregation by Non-Steroidal Anti-Inflammatory Compounds: A Structural and Thermodynamic Analysis

Posted: 06 Mar 2013 12:00 AM PST

Transthyretin (TTR) is a homotetrameric protein that circulates in plasma and cerebral spinal fluid (CSF) whose aggregation into amyloid fibrils has been associated with at least two different amyloid diseases: senile systemic amyloidosis (SSA) and familial amyloid polyneuropathy (FAP). In SSA aggregates are composed of WT-TTR, while in FAP more than 100 already-described variants have been found in deposits. Until now, TTR-related diseases have been untreatable, although a new drug called Tafamidis has been approved only in Europe to specifically treat V30M patients. Thus, new strategies are still necessary to treat FAP caused by other variants of TTR. TTR has two channels in the dimer interface that bind to the hormone thyroxin and that have been used to accommodate anti-amyloidogenic compounds. These compounds stabilize the tetramers, rendering TTR less amyloidogenic. Here, we investigated the effects of three non-steroidal anti-inflammatory compounds—sulindac (SUL), indomethacin (IND) and lumiracoxib (LUM)—as tetramer stabilizers and aggregation inhibitors. WT-TTR and the very aggressive TTR variant L55P were used as models. These compounds were able to stabilize TTR against high hydrostatic pressure (HHP), increasing the ΔGf by several kcal. They were also effective in inhibiting WT-TTR and L55P acid- or HHP-induced aggregation; in particular, LUM and IND were very effective, inhibiting almost 100% of the aggregation of both proteins under certain conditions. The species formed when aggregation was performed in the presence of these compounds were much less toxic to cells in culture. The crystal structures of WT-TTR bound to the three compounds were solved at high resolution, allowing the identification of the relevant protein:drug interactions. We discuss here the ligand-binding features of LUM, IND and SUL to TTR, emphasizing the critical interactions that render the protein more stable and less amyloidogenic.

Jujmp Rampage - Screenshots

Posted: 06 Mar 2013 02:24 AM PST

Jump Rampage is a story about what happens when an alien meets evil rabbits - and makes them angry. You are in control of a very jumpy alien who immediately faces trouble from all sides. Standing ruthlessly in his way are matrix-like evil rabbits, who dress in pink with tasteless sunglasses. These things are mean and they pack a punch, and must be avoided at all costs!

You must jump rapidly, use jetpacks, grab coins, and steal red power shoes, or it's all over. And here's a little secret - don't ever run into an evil rabbit without a good shield! Beware at all times: Jump Rampage is an iPhone experience that facilitates jump-based, coin-powered, Alien-To-Rabbit fight situations. Do not try this anywhere else, even if you have access to a real alien.

FEATURES
- Magic shoes enable Turbo Jumping!
- Jetpacks help you soar when you reach Impossible Platforms.
- Shields protect you from EVIL RABBITS.
- Coin Magnets make you rich and powerful in the store.
- The Coin Doubler doubles every coin you collect!
- Submit scores to Facebook. Beat your friends!
- Global Leaderboard and Achievements with Game Center.

IJMS, Vol. 14, Pages 5264-5283: Caffeic Acid Phenethyl Ester as a Potential Treatment for Advanced Prostate Cancer Targeting Akt Signaling

Posted: 06 Mar 2013 12:00 AM PST

Prostate cancer is the fifth most common cancer overall in the world. Androgen ablation therapy is the primary treatment for metastatic prostate cancer. However, most prostate cancer patients receiving the androgen ablation therapy ultimately develop recurrent castration-resistant tumors within 1–3 years after treatment. The median overall survival time is 1–2 years after tumor relapse. Chemotherapy shows little effect on prolonging survival for patients with metastatic hormone-refractory prostate cancer. More than 80% of prostate tumors acquire mutation or deletion of tumor suppressor phosphatase and tensin homolog (PTEN), a negative regulator of PI3K/Akt signaling, indicating that inhibition of PI3K/Akt might be a potential therapy for advanced prostate tumors. Caffeic acid phenethyl ester (CAPE) is a strong antioxidant extracted from honeybee hive propolis. CAPE is a well-known NF-κB inhibitor. CAPE has been used in folk medicine as a potent anti-inflammatory agent. Recent studies indicate that CAPE treatment suppresses tumor growth and Akt signaling in human prostate cancer cells. We discuss the potential of using CAPE as a treatment for patients with advanced prostate cancer targeting Akt signaling pathway in this review article.

Polymers, Vol. 5, Pages 269-283: Charge-Transfer Complexes Studied by Dynamic Force Spectroscopy

Posted: 06 Mar 2013 12:00 AM PST

In this paper, the strength and kinetics of two charge-transfer complexes, naphthol-methylviologen and pyrene-methylviologen, are studied using dynamic force spectroscopy. The dissociation rates indicate an enhanced stability of the pyrene-methylviologen complex, which agrees with its higher thermodynamic stability compared to naphthol-methylviologen complex.

Remote Sensing, Vol. 5, Pages 1204-1219: New Microslice Technology for Hyperspectral Imaging

Posted: 06 Mar 2013 12:00 AM PST

We present the results of a project to develop a proof of concept for a novel hyperspectral imager based on the use of advanced micro-optics technology. The technology gives considerably more spatial elements than a classic pushbroom which translates into far more light being integrated per unit of time. This permits us to observe at higher spatial and/or spectral resolution, darker targets and under lower illumination, as in the early morning. Observations of faint glow at night should also be possible but need further studies. A full instrument for laboratory demonstration and field tests has now been built and tested. It has about 10,000 spatial elements and spectra 150 pixel long. It is made of a set of cylindrical fore-optics followed by a new innovative optical system called a microslice Integral Field Unit (IFU) which is itself followed by a standard spectrograph. The fore-optics plus microslice IFU split the field into a large number of small slit-like images that are dispersed in the spectrograph. Our goal is to build instruments with at least hundreds of thousands of spatial elements.

Remote Sensing, Vol. 5, Pages 1177-1203: Trends and ENSO/AAO Driven Variability in NDVI Derived Productivity and Phenology alongside the Andes Mountains

Posted: 06 Mar 2013 12:00 AM PST

Increasing water use and droughts, along with climate variability and land use change, have seriously altered vegetation growth patterns and ecosystem response in several regions alongside the Andes Mountains. Thirty years of the new generation biweekly normalized difference vegetation index (NDVI3g) time series data show significant land cover specific trends and variability in annual productivity and land surface phenological response. Productivity is represented by the growing season mean NDVI values (July to June). Arid and semi-arid and sub humid vegetation types (Atacama desert, Chaco and Patagonia) across Argentina, northern Chile, northwest Uruguay and southeast Bolivia show negative trends in productivity, while some temperate forest and agricultural areas in Chile and sub humid and humid areas in Brazil, Bolivia and Peru show positive trends in productivity. The start (SOS) and length (LOS) of the growing season results show large variability and regional hot spots where later SOS often coincides with reduced productivity. A longer growing season is generally found for some locations in the south of Chile (sub-antarctic forest) and Argentina (Patagonia steppe), while central Argentina (Pampa-mixed grasslands and agriculture) has a shorter LOS. Some of the areas have significant shifts in SOS and LOS of one to several months. The seasonal Multivariate ENSO Indicator (MEI) and the Antarctic Oscillation (AAO) index have a significant impact on vegetation productivity and phenology in southeastern and northeastern Argentina (Patagonia and Pampa), central and southern Chile (mixed shrubland, temperate and sub-antarctic forest), and Paraguay (Chaco).

Molecules, Vol. 18, Pages 3028-3040: Biotransformation of Isoflavone Using Enzymatic Reactions

Posted: 06 Mar 2013 12:00 AM PST

The roles of cytochrome P450 monooxygenases (CYPs) from Streptomyces spp. which are called the "treasure islands" for natural products for medicine and antibiotics are not well understood. Substrate specificity studies on CYPs may give a solution for elucidation of their roles. Based on homology sequence information, the CYP105D7 of a soluble cytochrome P450 known as heme protein from Streptomyces avermitilis MA4680 was expressed using the T7 promoter of the bacterial expression vector pET24ma, over-expressed in Escherichia coli system and characterized. An engineered whole cell system for daidzein hydroxylation was constructed using an exogenous electron transport system from ferredoxin reductase (PdR) and ferredoxin (Pdx). Also, an in vitro reaction study showed the purified CYP105D7 enzyme, using NADH-dependent-reducing equivalents of a redox partner from Pseudomonas putida, hydroxylated daidzein at the 3' position of the B ring to produce 7,3,'4' trihydroxyisoflavone. The hydroxylated position was confirmed by GC-MS analysis. The turnover number of the enzyme was 0.69 μmol 7,3,'4'-trihydroxyisoflavone produced per μmol P450 per min. This enzyme CYP105D7 represents a novel type of 3'-hydroxylase for daidzein hydroxylation. A P450 inhibitor such as coumarin significantly (ca.98%) inhibited the daidzein hydroxylation activity.

Energies, Vol. 6, Pages 1497-1526: 2002–2012: 10 Years of Research Progress in Horizontal-Axis Marine Current Turbines

Posted: 06 Mar 2013 12:00 AM PST

Research in marine current energy, including tidal and ocean currents, has undergone significant growth in the past decade. The horizontal-axis marine current turbine is one of the machines used to harness marine current energy, which appears to be the most technologically and economically viable one at this stage. A number of large-scale marine current turbines rated at more than 1 MW have been deployed around the World. Parallel to the development of industry, academic research on horizontal-axis marine current turbines has also shown positive growth. This paper reviews previous research on horizontal-axis marine current turbines and provides a concise overview for future researchers who might be interested in horizontal-axis marine current turbines. The review covers several main aspects, such as: energy assessment, turbine design, wakes, generators, novel modifications and environmental impact. Future trends for research on horizontal-axis marine current turbines are also discussed.

Molecules, Vol. 18, Pages 3018-3027: Natural Aristolactams and Aporphine Alkaloids as Inhibitors of CDK1/Cyclin B and DYRK1A

Posted: 06 Mar 2013 12:00 AM PST

In an effort to find potent inhibitors of the protein kinases DYRK1A and CDK1/Cyclin B, a systematic in vitro evaluation of 2,500 plant extracts from New Caledonia and French Guyana was performed. Some extracts were found to strongly inhibit the activity of these kinases. Four aristolactams and one lignan were purified from the ethyl acetate extracts of Oxandra asbeckii and Goniothalamus dumontetii, and eleven aporphine alkaloids were isolated from the alkaloid extracts of Siparuna pachyantha, S. decipiens, S. guianensis and S. poeppigii. Among these compounds, velutinam, aristolactam AIIIA and medioresinol showed submicromolar IC50 values on DYRK1A.

Molbank, Vol. 2013, Article M796: 4'-Bromophenyl Triptycene-2,5-diol

Posted: 06 Mar 2013 12:00 AM PST

Diels Alder reaction of 4-bromophenylquinone (Br-PQ) with anthracene, followed by reduction affords the desired 4'-bromophenyl triptycene-2,5-diol (T-Br-PH). The described synthesis represents a simple and efficient method for the construction of a triptycene ring with a bromophenyl pendant. The intermediate and the final compound (T-Br-PH) have been characterized by elemental analysis, NMR, and LCMS techniques.

4 Pics 1 Word - Screenshots

Posted: 06 Mar 2013 01:44 AM PST

Features
PURE, INSTANT FUN - No registration, no complicated rules. Just start playing and have fun!
CAN YOU GUESS THEM ALL? - Can you guess all words and unlock all levels? Countless puzzles from easy to tricky are waiting for you!
SIMPLE AND HIGHLY ADDICTIVE GAMEPLAY - What is the word we are looking for? Look at the four pictures, find out what they have in common. Win!

Ku: Shroud of the Morrigan - Screenshots

Posted: 06 Mar 2013 01:15 AM PST

Forged from an ancient tale of Celtic mythology - a uniquely different recounting of the massive legendary epic, the Tain. An action-adventure set in a future Ireland devastated by technomagical war and economic collapse, humanity clings on by using the remnants of technology that survive.

Nanomaterials, Vol. 3, Pages 173-191: Liquid-State and Solid-State Properties of Nanotube/Polypropylene Nanocomposites Elaborated via a Simple Procedure

Posted: 06 Mar 2013 12:00 AM PST

Non-modified Multiwalled Carbon Nanotubes (MWCNT) and polypropylene (PP) in absence of compatibilizer have been chosen to elaborate MWCNT/PP nanocomposites using a simple melt-mixing dispersing method. Calorimetry results indicate little effect of MWCNTs on crystallinity of PP, revealing not much interaction between nanotubes and PP chains, which is compatible with the employed manufacturing procedure. In any case, a hindering of polymer chains motion by MWCNTs is observed in the molten state, using oscillatory flow experiments, and a rheological percolation threshold is determined. The percolation limit is not noticed by Pressure-Volume-Temperature (PVT) measurements in the melt, because this technique rather detects local motions. Keeping the nanocomposites in the molten state provokes an electrical conductivity increase of several orders of magnitude, but on ulterior crystallization, the conductivity decreases, probably due to a reduction of the ionic conductivity. For a concentration of 2% MWCNTs, in the limit of percolation, the conductivity decreases considerably more, because percolation network constituted in the molten state is unstable and is destroyed during crystallization.

Future Internet, Vol. 5, Pages 56-66: African Americans and Network Disadvantage: Enhancing Social Capital through Participation on Social Networking Sites

Posted: 06 Mar 2013 12:00 AM PST

This study examines the participation of African Americans on social networking sites (SNS), and evaluates the degree to which African Americans engage in activities in the online environment to mitigate social capital deficits. Prior literature suggests that compared with whites, African Americans have less social capital that can enhance their socio-economic mobility. As such, my research question is: do African Americans enhance their social capital through their participation on SNS? I use nationally representative data collected from the Pew Internet and American Life Project to explore the research question. The results suggest that the online environment is potentially a space in which African Americans can lessen social capital deficits.

Marine Drugs, Vol. 11, Pages 655-679: Biochemical and Electrophysiological Characterization of Two Sea Anemone Type 1 Potassium Toxins from a Geographically Distant Population of Bunodosoma caissarum

Posted: 06 Mar 2013 12:00 AM PST

Sea anemone (Cnidaria, Anthozoa) venom is an important source of bioactive compounds used as tools to study the pharmacology and structure-function of voltage-gated K+ channels (KV). These neurotoxins can be divided into four different types, according to their structure and mode of action. In this work, for the first time, two toxins were purified from the venom of Bunodosoma caissarum population from Saint Peter and Saint Paul Archipelago, Brazil. Sequence alignment and phylogenetic analysis reveals that BcsTx1 and BcsTx2 are the newest members of the sea anemone type 1 potassium channel toxins. Their functional characterization was performed by means of a wide electrophysiological screening on 12 different subtypes of KV channels (KV1.1–KV1.6; KV2.1; KV3.1; KV4.2; KV4.3; hERG and Shaker IR). BcsTx1 shows a high affinity for rKv1.2 over rKv1.6, hKv1.3, Shaker IR and rKv1.1, while Bcstx2 potently blocked rKv1.6 over hKv1.3, rKv1.1, Shaker IR and rKv1.2. Furthermore, we also report for the first time a venom composition and biological activity comparison between two geographically distant populations of sea anemones.
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