понедельник, 19 декабря 2011 г.

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Viruses, Vol. 3, Pages 2442-2461: The Human Lung Adenocarcinoma Cell Line EKVX Produces an Infectious Xenotropic Murine Leukemia Virus

Posted: 19 Dec 2011 12:00 AM PST

The cell lines of the NCI-60 panel represent different cancer types and have been widely utilized for drug screening and molecular target identification. Screening these cell lines for envelope proteins or gene sequences related to xenotropic murine leukemia viruses (X-MLVs) revealed that one cell line, EKVX, was a candidate for production of an infectious gammaretrovirus. The presence of a retrovirus infectious to human cells was confirmed by the cell-free transmission of infection to the human prostate cancer cell line LNCaP. Amplification and sequencing of additional proviral sequences from EKVX confirmed a high degree of similarity to X-MLV. The cell line EKVX was established following passage of the original tumor cells through nude mice, providing a possible source of the X-MLV found in the EKVX cells.

Newt's Nudes: Renaissance paintings with Newt Gingrich's head

Posted: 19 Dec 2011 02:59 AM PST

Newt's Nudes imagines a world where Newt Gingrich attends decadent feasts, consoles distressed Biblical characters, and occasionally, as a cherub, saucily peers down on amply-bosomed maidens. Perhaps not that far off from real life!

Newt's Nudes Thanks, pjtrimble!

Toxins, Vol. 3, Pages 1518-1553: Current and Future Experimental Strategies for Structural Analysis of Trichothecene Mycotoxins—A Prospectus

Posted: 19 Dec 2011 12:00 AM PST

Fungal toxins, such as those produced by members of the order Hypocreales, have widespread effects on cereal crops, resulting in yield losses and the potential for severe disease and mortality in humans and livestock. Among the most toxic are the trichothecenes. Trichothecenes have various detrimental effects on eukaryotic cells including an interference with protein production and the disruption of nucleic acid synthesis. However, these toxins can have a wide range of toxicity depending on the system. Major differences in the phytotoxicity and cytotoxicity of these mycotoxins are observed for individual members of the class, and variations in toxicity are observed among different species for each individual compound. Furthermore, while diverse toxicological effects are observed throughout the whole cellular system upon trichothecene exposure, the mechanism of toxicity is not well understood. In order to comprehend how these toxins interact with the cell, we must first have an advanced understanding of their structure and dynamics. The structural analysis of trichothecenes was a subject of major interest in the 1980s, and primarily focused on crystallographic and solution-state Nuclear Magnetic Resonance (NMR) spectroscopic studies. Recent advances in structural determination through solution- and solid-state NMR, as well as computation based molecular modeling is leading to a resurgent interest in the structure of these and other mycotoxins, with the focus shifting in the direction of structural dynamics. The purpose of this work is to first provide a brief overview of the structural data available on trichothecenes and a characterization of the methods commonly employed to obtain such information. A summary of the current understanding of the relationship between structure and known function of these compounds is also presented. Finally, a prospectus on the application of new emerging structural methods on these and other related systems is discussed.

Sensors, Vol. 11, Pages 11809-11832: Surface Acoustic Wave (SAW) Vibration Sensors

Posted: 19 Dec 2011 12:00 AM PST

In the paper a feasibility study on the use of surface acoustic wave (SAW) vibration sensors for electronic warning systems is presented. The system is assembled from concatenated SAW vibration sensors based on a SAW delay line manufactured on a surface of a piezoelectric plate. Vibrations of the plate are transformed into electric signals that allow identification of the sensor and localization of a threat. The theoretical study of sensor vibrations leads us to the simple isotropic model with one degree of freedom. This model allowed an explicit description of the sensor plate movement and identification of the vibrating sensor. Analysis of frequency response of the ST-cut quartz sensor plate and a damping speed of its impulse response has been conducted. The analysis above was the basis to determine the ranges of parameters for vibrating plates to be useful in electronic warning systems. Generally, operation of electronic warning systems with SAW vibration sensors is based on the analysis of signal phase changes at the working frequency of delay line after being transmitted via two circuits of concatenated four-terminal networks. Frequencies of phase changes are equal to resonance frequencies of vibrating plates of sensors. The amplitude of these phase changes is proportional to the amplitude of vibrations of a sensor plate. Both pieces of information may be sent and recorded jointly by a simple electrical unit.

Energies, Vol. 4, Pages 2249-2272: Study on the Decomposition of Factors Affecting Energy-Related Carbon Emissions in Guangdong Province, China

Posted: 19 Dec 2011 12:00 AM PST

Guangdong is China's largest province in terms of energy consumption. The energy-related carbon emissions in Guangdong province are calculated, and two extended and improved decomposition models for energy-related carbon emissions are established with the Logarithmic Mean Divisia Index method based on the basic principle of Kaya identity. Main results are as follows: (1) the energy-related carbon emissions from the three strata of industry, except the primary industry, and household energy consumption in Guangdong province show increasing trend from 1995 to 2009; (2) the main driving and inhibiting factors which influence energy-related carbon emissions are economic output and energy intensity, respectively, while the contributions of energy mix, industrial structures, population size and living standards are not significant during the period of interest. It is concluded that optimizing the energy mix by exploiting new energy sources and cutting down energy intensity by developing low-carbon technologies are the two most effective approaches to reduce carbon emissions for Guangdong province in the future. The results and proposals in this paper provided reference for relevant administrative departments in the Government of Guangdong province to develop policies for energy conservation and emission reduction as well as to promote development of low-carbon economy.

Sensors, Vol. 11, Pages 11774-11808: Electromagnetic Imaging Methods for Nondestructive Evaluation Applications

Posted: 19 Dec 2011 12:00 AM PST

Electromagnetic nondestructive tests are important and widely used within the field of nondestructive evaluation (NDE). The recent advances in sensing technology, hardware and software development dedicated to imaging and image processing, and material sciences have greatly expanded the application fields, sophisticated the systems design and made the potential of electromagnetic NDE imaging seemingly unlimited. This review provides a comprehensive summary of research works on electromagnetic imaging methods for NDE applications, followed by the summary and discussions on future directions.

IJMS, Vol. 12, Pages 9471-9480: Molecular Diagnosis of 5α-Reductase Type II Deficiency in Brazilian Siblings with 46,XY Disorder of Sex Development

Posted: 19 Dec 2011 12:00 AM PST

The steroid 5α-reductase type II enzyme catalyzes the conversion of testosterone (T) to dihydrotestosterone (DHT), and its deficiency leads to undervirilization in 46,XY individuals, due to an impairment of this conversion in genital tissues. Molecular analysis in the steroid 5α-reductase type II gene (SRD5A2) was performed in two 46,XY female siblings. SRD5A2 gene sequencing revealed that the patients were homozygous for p.Gln126Arg missense mutation, which results from the CGA > CAA nucleotide substitution. The molecular result confirmed clinical diagnosis of 46,XY disorder of sex development (DSD) for the older sister and directed the investigation to other family members. Studies on SRD5A2 protein structure showed severe changes at NADPH binding region indicating that structural modeling analysis can be useful to evaluate the deleterious role of a mutation as causing 5α-reductase type II enzyme deficiency.

Windosill (iPad)

Posted: 18 Dec 2011 06:12 PM PST

[Platform: Mobile (iPad (iOS))] The chugging little car is back! Windosill, a 2009 release from Vector Park, creator of Acrobots, Levers, and Feed the Head, has wormed its way to the iPad, bringing with it all the dream-like levels of the original browser game. Windosill remains one of the most charming experiences any casual player could hope to stumble upon, and it's a perfect fit for the iPad and a great mobile game as well!

IJMS, Vol. 12, Pages 9463-9470: Use of Peroxyacetic Acid as Green Chemical on Yield and Sensorial Quality in Watercress (Nasturtium officinale R. Br.) Under Soilless Culture

Posted: 19 Dec 2011 12:00 AM PST

The goal of this research was to evaluate the effect of different doses of peroxyacetic acid on the productivity of watercress (Nasturtium officinale R. Br.) cultivated hydroponically using a constant nutritive solution. Green chemistry in protected horticulture seeks compatibility with the environment through the creation of biodegradable byproducts. In hydroponics, appropriate doses of peroxyacetic mixtures deliver these byproducts while also oxygenating the roots. Watercress producers who recirculate the nutritive solution can use these mixtures in order to increase oxygenation in the hydroponic system. The experiment took place between August and December 2009, beginning with the planting of the watercress seeds and concluding with the completion of the sensory panels. A completely random design was used, including three treatments and four repetitions, with applications of 0, 20 and 40 mg L−1 of the peroxyacetic mixture. Measured variables were growth (plant height, leaf length and stem diameter), yield (weight per plant and dry matter) and organoleptic quality (color and sensory panel). The application of 40 mg L−1 of the peroxyacetic mixture had a greater effect on the growth and development of the plants, which reached an average height of 29.3 cm, stem diameter of 3.3 mm and leaf length of 7.6 cm, whereas the control group reached an average height of only 20.2 cm, stem diameter of 1.9 mm and leaf length of 5.7 cm. The application of the peroxyacetic mixtures resulted in an improvement in growth parameters as well as in yield. Individual weights achieved using the 40 mg L−1 dose were 1.3 g plant−1 in the control group and 3.4 g plant−1 in the experimental group (62% yield increase). Sensory analysis revealed no differences in organoleptic quality.

Polymers, Vol. 3, Pages 2142-2155: Plasma Treated Multi-Walled Carbon Nanotubes (MWCNTs) for Epoxy Nanocomposites

Posted: 19 Dec 2011 12:00 AM PST

Plasma nanocoating of allylamine were deposited on the surfaces of multi-walled carbon nanotubes (MWCNTs) to provide desirable functionalities and thus to tailor the surface characteristics of MWCNTs for improved dispersion and interfacial adhesion in epoxy matrices. Plasma nanocoated MWCNTs were characterized using scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), surface contact angle, and pH change measurements. Mechanical testing results showed that epoxy reinforced with 1.0 wt % plasma coated MWCNTs increased the tensile strength by 54% as compared with the pure epoxy control, while epoxy reinforced with untreated MWCNTs have lower tensile strength than the pure epoxy control. Optical and electron microscopic images show enhanced dispersion of plasma coated MWCNTs in epoxy compared to untreated MWCNTs. Plasma nanocoatings from allylamine on MWCNTs could significantly enhance their dispersion and interfacial adhesion in epoxy matrices. Simulation results based on the shear-lag model derived from micromechanics also confirmed that plasma nanocoating on MWCNTs significantly improved the epoxy/fillers interface bonding and as a result the increased composite strength.

IJERPH, Vol. 8, Pages 4729-4745: Determining the Maximum Cumulative Ratios for Mixtures Observed in Ground Water Wells Used as Drinking Water Supplies in the United States

Posted: 19 Dec 2011 12:00 AM PST

The maximum cumulative ratio (MCR) developed in previous work is a tool to evaluate the need to perform cumulative risk assessments. MCR is the ratio of the cumulative exposures to multiple chemicals to the maximum exposure from one of the chemicals when exposures are described using a common metric. This tool is used to evaluate mixtures of chemicals measured in samples of untreated ground water as source for drinking water systems in the United States. The mixtures of chemicals in this dataset differ from those examined in our previous work both in terms of the predicted toxicity and compounds measured. Despite these differences, MCR values in this study follow patterns similar to those seen earlier. MCR values for the mixtures have a mean (range) of 2.2 (1.03–5.4) that is much smaller than the mean (range) of 16 (5–34) in the mixtures in previous study. The MCR values of the mixtures decline as Hazard Index (HI) values increase. MCR values for mixtures with larger HI values are not affected by possible contributions from chemicals that may occur at levels below the detection limits. This work provides a second example of use of the MCR tool in the evaluation of mixtures that occur in the environment.

IJMS, Vol. 12, Pages 9440-9462: Dynamic Structure-Based Pharmacophore Model Development: A New and Effective Addition in the Histone Deacetylase 8 (HDAC8) Inhibitor Discovery

Posted: 19 Dec 2011 12:00 AM PST

Histone deacetylase 8 (HDAC8) is an enzyme involved in deacetylating the amino groups of terminal lysine residues, thereby repressing the transcription of various genes including tumor suppressor gene. The over expression of HDAC8 was observed in many cancers and thus inhibition of this enzyme has emerged as an efficient cancer therapeutic strategy. In an effort to facilitate the future discovery of HDAC8 inhibitors, we developed two pharmacophore models containing six and five pharmacophoric features, respectively, using the representative structures from two molecular dynamic (MD) simulations performed in Gromacs 4.0.5 package. Various analyses of trajectories obtained from MD simulations have displayed the changes upon inhibitor binding. Thus utilization of the dynamically-responded protein structures in pharmacophore development has the added advantage of considering the conformational flexibility of protein. The MD trajectories were clustered based on single-linkage method and representative structures were taken to be used in the pharmacophore model development. Active site complimenting structure-based pharmacophore models were developed using Discovery Studio 2.5 program and validated using a dataset of known HDAC8 inhibitors. Virtual screening of chemical database coupled with drug-like filter has identified drug-like hit compounds that match the pharmacophore models. Molecular docking of these hits reduced the false positives and identified two potential compounds to be used in future HDAC8 inhibitor design.

Marine Drugs, Vol. 9, Pages 2773-2792: Natural Product Chemistry of Gorgonian Corals of Genus Junceella—Part II

Posted: 19 Dec 2011 12:00 AM PST

The structures, names, bioactivities, and references of 81 new secondary metabolites obtained from gorgonian corals belonging to the genus Junceella are described in this review. All compounds mentioned in this review were obtained from sea whip gorgonian corals Junceella fragilis and Junceella juncea, collected from the tropical and subtropical Indo-Pacific Ocean.

Molecules, Vol. 16, Pages 10645-10652: Four Eremophil-9-en-8-one Derivatives from Cremanthodium stenactinium Samples Collected in China

Posted: 19 Dec 2011 12:00 AM PST

Two samples of Cremanthodium stenactinium (Asteraceae) were collected in Sichuan Province, China. From the ethyl acetate extracts of the roots, three new eremophilane-type sesquiterpenoids and one new trinoreremophilane compound were isolated, together with other known eremophilanes. Their structures were determined based on the spectroscopic data. This is the first report of isolation of eremophilane-type compounds from the genus Cremanthodium.

Entropy, Vol. 13, Pages 2024-2035: Fluctuation, Dissipation and the Arrow of Time

Posted: 19 Dec 2011 12:00 AM PST

The recent development of the theory of fluctuation relations has led to new insights into the ever-lasting question of how irreversible behavior emerges from time-reversal symmetric microscopic dynamics. We provide an introduction to fluctuation relations, examine their relation to dissipation and discuss their impact on the arrow of time question.

Molecules, Vol. 16, Pages 10631-10644: Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Cultures by Oligosaccharides from Its Endophytic Fungus Fusarium oxysporum Dzf17

Posted: 19 Dec 2011 12:00 AM PST

The effects of the oligosaccharides from the endophytic fungus Fusarium oxysporum Dzf17 as elicitors on diosgenin production in cell suspension cultures of its host Dioscorea zingiberensis were investigated. Three oligosaccharides, DP4, DP7 and DP10, were purified from the oligosaccharide fractions DP2-5, DP5-8 and DP8-12, respectively, which were prepared from the water-extracted mycelial polysaccharide of the endophytic fungus F. oxysporum Dzf17. When the cell cultures were treated with fraction DP5-8 at 20 mg/L on day 26 and harvested on day 32, the maximum diosgenin yield (2.187 mg/L) was achieved, which was 5.65-fold of control (0.387 mg/L). When oligosaccharides DP4, DP7 and DP10 were individually added to 26-day-old D. zingiberensis cell cultures at concentrations of 2, 4, 6, 8 and 10 mg/L in medium, DP7 at 6 mg/L was found to significantly enhance diosgenin production, with a yield of 3.202 mg/L, which was 8.27-fold of control. When the cell cultures were treated with DP7 twice on days 24 and 26, and harvested on day 30, both diosgenin content and yield were significantly increased and reached the maximums of 1.159 mg/g dw and 4.843 mg/L, both of which were higher than those of single elicitation, and were 9.19- and 12.38-fold of control, respectively.

Entropy, Vol. 13, Pages 2013-2023: Coincidences and Estimation of Entropies of Random Variables with Large Cardinalities

Posted: 19 Dec 2011 12:00 AM PST

We perform an asymptotic analysis of the NSB estimator of entropy of a discrete random variable. The analysis illuminates the dependence of the estimates on the number of coincidences in the sample and shows that the estimator has a well defined limit for a large cardinality of the studied variable. This allows estimation of entropy with no a priori assumptions about the cardinality. Software implementation of the algorithm is available.

Molecules, Vol. 16, Pages 10619-10630: The in Vitro Structure-Related Anti-Cancer Activity of Ginsenosides and Their Derivatives

Posted: 19 Dec 2011 12:00 AM PST

Panax ginseng has long been used in Asia as a herbal medicine for the prevention and treatment of various diseases, including cancer. The current study evaluated the cytotoxic potency against a variety of cancer cells by using ginseng ethanol extracts (RSE), protopanaxadiol (PPD)-type, protopanaxatriol (PPT)-type ginsenosides fractions, and their hydrolysates, which were prepared by stepwise hydrolysis of the sugar moieties of the ginsenosides. The results showed that the cytotoxic potency of the hydrolysates of RSE and total PPD-type or PPT-type ginsenoside fractions was much stronger than the original RSE and ginsenosides; especially the hydrolysate of PPD-type ginsenoside fractions. Subsequently, two derivatives of protopanaxadiol (1), compounds 2 and 3, were synthesized via hydrogenation and dehydration reactions of compound 1. Using those two derivatives and the original ginsenosides, a comparative study on various cancer cell lines was conducted; the results demonstrated that the cytotoxic potency was generally in the descending order of compound 3 > 20(S)-dihydroprotopanaxadiol (2) > PPD (1) > 20(S)-Rh2 > 20(R)-Rh2 ≈ 20(R)-Rg3 ≈ 20(S)-Rg3. The results clearly indicate the structure-related activities in which the compound with less polar chemical structures possesses higher cytotoxic activity towards cancer cells.

IJERPH, Vol. 8, Pages 4714-4728: Perceptions of Heat-Susceptibility in Older Persons: Barriers to Adaptation

Posted: 19 Dec 2011 12:00 AM PST

The increase in the frequency of very hot weather that is a predicted consequence of climate change poses an emerging threat to public health. Extreme heat can be harmful to the health of older persons who are known to be amongst the most vulnerable in the community. This study aimed to investigate factors influencing the ability of older persons to adapt to hot conditions, and barriers to adaptation. A qualitative study was conducted in Adelaide, Australia, involving focus groups and interviews with stakeholders including key personnel involved in aged care, community services, government sectors, emergency services and policy making. Findings revealed a broad range of factors that underpin the heat-susceptibility of the aged. These were categorized into four broad themes relating to: physiology and an age-related decline in health; socioeconomic factors, particularly those influencing air conditioning use; psychological issues including fears and anxieties about extreme heat; and adaptive strategies that could be identified as both enablers and barriers. As a consequence, the ability and willingness to undertake behavior change during heatwaves can therefore be affected in older persons. Additionally, understanding the control panels on modern air conditioners can present challenges for the aged. Improving heat-health knowledge and addressing the social and economic concerns of the older population will assist in minimizing heat-related morbidity and mortality in a warming climate.

Molecules, Vol. 16, Pages 10604-10618: Terpenoids from Endophytic Fungi

Posted: 19 Dec 2011 12:00 AM PST

This work reviews the production of terpenoids by endophytic fungi and their biological activities, in period of 2006 to 2010. Sixty five sesquiterpenes, 45 diterpenes, five meroterpenes and 12 other terpenes, amounting to 127 terpenoids were isolated from endophytic fungi.
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