Games |
- Youda Mystery: The Stanwick Legacy 1.4 - Hidden object game with a mystery theme.. (Demo)
- Chromium 17.0.963.56 - Fast and stable open-source browser.. (Free)
- FLV Spider 3.0.6 - Capture online video, save it to Mac and iPod.. (Shareware)
- IJMS, Vol. 13, Pages 2133-2147: cDNA Cloning, Overexpression, Purification and Pharmacologic Evaluation for Anticancer Activity of Ribosomal Protein L23A Gene (RPL23A) from the Giant Panda
- IJMS, Vol. 13, Pages 2119-2132: Correlation Coefficients Between Different Methods of Expressing Bacterial Quantification Using Real Time PCR
- IJMS, Vol. 13, Pages 2110-2118: The Effect of Toll-Like Receptor 4 in the Aqueous Humor of Endotoxin-Induced Uveitis
- xStand 6.5.1 - Full screen web browser for internet kiosks.. (Demo)
- Socialite 1.4.0 - Creates a single interface for multiple social websites.. (Shareware)
- xScan 3.1.2 - Monitor the behavior and health of your Mac.. (Demo)
- IJMS, Vol. 13, Pages 2091-2109: Antioxidant-Induced Stress
- Marine Drugs, Vol. 10, Pages 465-476: Anti-Melanogenic Property of Geoditin A in Murine B16 Melanoma Cells
- Marine Drugs, Vol. 10, Pages 451-464: Novel Marine Phenazines as Potential Cancer Chemopreventive and Anti-Inflammatory Agents
- Marine Drugs, Vol. 10, Pages 439-450: Steroids from the Soft Coral Sinularia crassa
- Pharmaceuticals, Vol. 5, Pages 236-248: Probiotic Ferulic Acid Esterase Active Lactobacillus fermentum NCIMB 5221 APA Microcapsules for Oral Delivery: Preparation and in Vitro Characterization
- Pharmaceuticals, Vol. 5, Pages 209-235: Structure Based Antibody-Like Peptidomimetics
- Pharmaceutics, Vol. 4, Pages 179-196: Preparation and Characterization of Amino Acids-Based Trimethoprim Salts
- Polymers, Vol. 4, Pages 561-589: Versatile Route to Synthesize Heterobifunctional Poly(ethylene glycol) of Variable Functionality for Subsequent Pegylation
- Polymers, Vol. 4, Pages 539-560: Antimicrobial Peptides: Multifunctional Drugs for Different Applications
- Resistance 3 OST now available
- The HotSpot - PlayStation Vita - 2/15/12
| Youda Mystery: The Stanwick Legacy 1.4 - Hidden object game with a mystery theme.. (Demo) Posted: 16 Feb 2012 02:39 AM PST  Youda Mystery: The Stanwick Legacy is the story of little Emma Stanwick. After her parents mysteriously deceased when she was still a little child, her grandfather sent Emma to a boarding school. Stanwick Manor, once a beautiful house and Emma's home, is now abandoned and empty. The manor has been in the family for ages. Emma's great-grandfather also lived here before she was born. She recalls her grandfather telling her that he was an inventor of some sorts: "A bit of a mysterious man." Recently her granddad died and she, being the last heir, inherits the manor. With mixed feelings about it all, she goes back to her old home to find out if there is still any secret to reveal. Did her granddad tell the full story? When her taxi approaches the house there is a strange light glowing through the windows. As Emma enters the mysterious manor she hears a strange heartbeat like sound. What's going on? During her search, Emma discovers that nothing is what it seems to be. Why is the fireplace lit? What is the purpose of the strange piping, running through the house? Why do some of the paintings move and what makes that heartbeat like sound? Emma's memories of living in the manor make it possible to open secret locks and lead her further into the house, revealing more and more secrets. Did grandfather leave enough clues for Emma to reveal the real Stanwick Legacy and will she be clever enough to do the right thing? Features:
Version 1.4: Release notes were unavailable when this listing was updated. Mac OS X 10.4 or later Download Now This posting includes an audio/video/photo media file: Download Now |
| Chromium 17.0.963.56 - Fast and stable open-source browser.. (Free) Posted: 16 Feb 2012 02:45 AM PST  Chromium is an open-source browser project that aims to build a safer, faster, and more stable way for all Internet users to experience the web. FreeSMUG-Free OpenSource Mac User Group build is based on the stable Chrome code plus "Check for Updates..." command.
Mac OS X 10.5 or later Download Now This posting includes an audio/video/photo media file: Download Now |
| FLV Spider 3.0.6 - Capture online video, save it to Mac and iPod.. (Shareware) Posted: 16 Feb 2012 03:07 AM PST  FLV Spider is a simple utility that allows you to capture online videos. So, if you see a cool online video, and would like to save it to your Mac or to your iPod, nothing is easier! Just run FLV Spider before you start to surf the Web. FLV Spider will automatically capture all online videos, opened in a web browser. Embedded Lossless Movie Trimmer lets you easily cut-off a portion of captured video, and Lossless Movie Joiner lets you easily join captured video/audio files into one continuous movie. Please read the help manual to learn more about these advanced tools. Version 3.0.6:
Mac OS X 10.4 or later Download Now |
| Posted: 16 Feb 2012 12:00 AM PST RPL23A gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L23P family of ribosomal proteins, which is located in the cytoplasm. The purpose of this paper was to explore the structure and anti-cancer function of ribosomal protein L23A (RPL23A) gene of the Giant Panda (Ailuropoda melanoleuca). The cDNA of RPL23A was cloned successfully from the Giant Panda using RT-PCR technology. We constructed a recombinant expression vector containing RPL23A cDNA and over-expressed it in Escherichia coli using pET28a plasmids. The expression product obtained was purified by using Ni chelating affinity chromatography. Recombinant protein of RPL23A obtained from the experiment acted on Hep-2 cells and human HepG-2 cells, then the growth inhibitory effect of these cells was observed by MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) assay. The result indicated that the length of the fragment cloned is 506 bp, and it contains an open-reading frame (ORF) of 471 bp encoding 156 amino acids. Primary structure analysis revealed that the molecular weight of the putative RPL23A protein is 17.719 kDa with a theoretical pI 11.16. The molecular weight of the recombinant protein RPL23A is 21.265 kDa with a theoretical pI 10.57. The RPL23A gene can be really expressed in E. coli and the RPL23A protein, fusioned with the N-terminally His-tagged protein, gave rise to the accumulation of an expected 22 KDa polypeptide. The data showed that the recombinant protein RPL23A had a time- and dose-dependency on the cell growth inhibition rate. The data also indicated that the effect at low concentrations was better than at high concentrations on Hep-2 cells, and that the concentration of 0.185 μg/mL had the best rate of growth inhibition of 36.31%. All results of the experiment revealed that the recombinant protein RPL23A exhibited anti-cancer function on the Hep-2 cells. The study provides a scientific basis and aids orientation for the research and development of cancer protein drugs as well as possible anti-cancer mechanisms. Further research is on going to determine the bioactive principle(s) of recombinant protein RPL23A responsible for its anticancer activity. |
| Posted: 16 Feb 2012 12:00 AM PST The applications of conventional culture-dependent assays to quantify bacteria populations are limited by their dependence on the inconsistent success of the different culture-steps involved. In addition, some bacteria can be pathogenic or a source of endotoxins and pose a health risk to the researchers. Bacterial quantification based on the real-time PCR method can overcome the above-mentioned problems. However, the quantification of bacteria using this approach is commonly expressed as absolute quantities even though the composition of samples (like those of digesta) can vary widely; thus, the final results may be affected if the samples are not properly homogenized, especially when multiple samples are to be pooled together before DNA extraction. The objective of this study was to determine the correlation coefficients between four different methods of expressing the output data of real-time PCR-based bacterial quantification. The four methods were: (i) the common absolute method expressed as the cell number of specific bacteria per gram of digesta; (ii) the Livak and Schmittgen, ΔΔCt method; (iii) the Pfaffl equation; and (iv) a simple relative method based on the ratio of cell number of specific bacteria to the total bacterial cells. Because of the effect on total bacteria population in the results obtained using ΔCt-based methods (ΔΔCt and Pfaffl), these methods lack the acceptable consistency to be used as valid and reliable methods in real-time PCR-based bacterial quantification studies. On the other hand, because of the variable compositions of digesta samples, a simple ratio of cell number of specific bacteria to the corresponding total bacterial cells of the same sample can be a more accurate method to quantify the population. |
| Posted: 16 Feb 2012 12:00 AM PST In our previous study, we found that acute anterior uveitis (AAU) could be induced in wild-type mice (C3H/HeN), but it could not be induced in TLR4 gene-deficient mice (C3H/HeJ), we concluded that the translocation of transcription factor nuclear factor-κB (NF-κB) may play an important role. In this study, we examined the concentration of different cytokines in the aqueous humor of C3H/HeN mice and C3H/HeJ mice with the aim of exploring the role of different cytokines in the lipopolysaccharide (LPS) and TLR4-mediated signal transduction in the development of AAU. |
| xStand 6.5.1 - Full screen web browser for internet kiosks.. (Demo) Posted: 16 Feb 2012 02:21 AM PST  xStand (formerly wKiosk) is a web browser designed to present web-based content or run web applications in a safe and stable way. Mostly used for public access terminals in museums or libraries, xStand can also be used as the default web browser for all members of a company or an organization. xStand allows you to restrict access to some web sites, the operating system, system settings, the downloading of files and applications. The computer is protected against any manipulation from users. xStand reinforce the productivity by disabling the access to distracting websites.. Since xStand is built with the Apple Safari Engine also named WebKit, users can benefit from all the features that this modern technology has to offer. xStand was developed with the intention to create a browser that would provide the same ease of use as other browsers, while adding maximum security and protection from user interference.. xStand has a wide range of additional functions which will allow you to customize your kiosk application to suit your individual needs and requirements. All you have to do is to decide what content you want to display and leave the rest to xStand. No programming skills are necessary. Configuring it can be accomplished within a couple of minutes. You can even add a touchscreen and enable the new virtual keyboard to entry information. xStand guarantees the operation and protection of your systems 24/7. Version 6.5.1:
Mac OS X 10.6.8 or later Download Now This posting includes an audio/video/photo media file: Download Now |
| Socialite 1.4.0 - Creates a single interface for multiple social websites.. (Shareware) Posted: 16 Feb 2012 02:13 AM PST  Socialite is a revolutionary social application. It seamlessly integrates many social websites, including Flickr, Facebook, Twitter, Reddit, RSS and Google Reader. It allows you to interact with them and receive notifications, all from a stunning user interface. Version 1.4.0: IMPORTANT: Dropped support for Mac OS X 10.5 Leopard. If you're on anything earlier than 10.6 don't update!
Mac OS X 10.6 or later Download Now This posting includes an audio/video/photo media file: Download Now |
| xScan 3.1.2 - Monitor the behavior and health of your Mac.. (Demo) Posted: 16 Feb 2012 02:19 AM PST  xScan (formerly CheckUp) has been specifically designed to help you monitor the behavior and health of your Mac in a simple and straightforward manner. This is done in real time. No more needing to go 'beneath the hood' to find out all those technical details that could be affecting your system. We've done all the work for you! xScan is able to detect common problems with a Mac and OS X, and will also provide useful tips to help you fix them. It's as easy as looking at the dashboard of your car! The first thing you will likely notice about xScan is the minimal interface. Upon opening xScan, you will be greeted by a simple black Toolbar down the right-hand side of your screen. That is all. This enables you to manage your Mac's system with as little interruption to your work as possible. From the preferences, you can control which side of the screen the Toolbar appears on, and also adjust the Toolbar's Transparency. A powerful feature of xScan is its ability to alert you to potential problems with your system. xScan is able to detect any hardware failures on your Mac. Recent Macs have sensors integrated into the hardware that xScan uses to assess the state of your system. It can also detect the health of all connected disks and will check if there are any major issues. Thus, xScan is a great solution in keeping an eye on your Mac! Version 3.1.2:
Mac OS X 10.6.8 or later Download Now This posting includes an audio/video/photo media file: Download Now |
| IJMS, Vol. 13, Pages 2091-2109: Antioxidant-Induced Stress Posted: 16 Feb 2012 12:00 AM PST Antioxidants are among the most popular health-protecting products, sold worldwide without prescription. Indeed, there are many reports showing the benefits of antioxidants but only a few questioning the possible harmful effects of these "drugs". The normal balance between antioxidants and free radicals in the body is offset when either of these forces prevails. The available evidence on the harmful effects of antioxidants is analyzed in this review. In summary, a hypothesis is presented that "antioxidant-induced stress" results when antioxidants overwhelm the body's free radicals. |
| Posted: 16 Feb 2012 12:00 AM PST Geoditin A, an isomalabaricane triterpene isolated from marine sponge Geodia japonica, has been demonstrated to induce apoptosis in leukemia HL60 cells and human colon HT29 cancer cells through an oxidative stress, a process also interfering with normal melanogenesis in pigment cells. Treatment of murine melanoma B16 cells with geoditin A decreased expression of melanogenic proteins and cell melanogenesis which was aggravated with adenylate cyclase inhibitor SQ22536, indicating melanogenic inhibition was mediated through a cAMP-dependent signaling pathway. Immunofluorescence microscopy and glycosylation studies revealed abnormal glycosylation patterns of melanogenic proteins (tyrosinase and tyrosinase-related protein 1), and a co-localization of tyrosinase with calnexin (CNX) and lysosome-associated membrane protein 1 (LAMP-1), implicating a post-translational modification in the ER and a degradation of tyrosinase in the lysosome. Taken together, potent anti-melanogenic property and the relatively low cytotoxicity of geoditin A have demonstrated its therapeutic potential as a skin lightening agent. |
| Posted: 16 Feb 2012 12:00 AM PST Two new (1 and 2) and one known phenazine derivative (lavanducyanin, 3) were isolated and identified from the fermentation broth of a marine-derived Streptomyces sp. (strain CNS284). In mammalian cell culture studies, compounds 1, 2 and 3 inhibited TNF-α-induced NFκB activity (IC50 values of 4.1, 24.2, and 16.3 μM, respectively) and LPS-induced nitric oxide production (IC50 values of >48.6, 15.1, and 8.0 μM, respectively). PGE2 production was blocked with greater efficacy (IC50 values of 7.5, 0.89, and 0.63 μM, respectively), possibly due to inhibition of cyclooxygenases in addition to the expression of COX-2. Treatment of cultured HL-60 cells led to dose-dependent accumulation in the subG1 compartment of the cell cycle, as a result of apoptosis. These data provide greater insight on the biological potential of phenazine derivatives, and some guidance on how various substituents may alter potential anti-inflammatory and anti-cancer effects. |
| Marine Drugs, Vol. 10, Pages 439-450: Steroids from the Soft Coral Sinularia crassa Posted: 16 Feb 2012 12:00 AM PST One new sterol, crassarosterol A (1), and four new steroidal glycosides, crassarosterosides A–D (2–5) were isolated from the Formosan soft coral Sinularia crassa. The absolute configuration of 1 was determined using the Mosher's method. The absolute configurations for the sugar moieties of 2–5 were determined by HPLC analysis on the o-tolylthiocarbamates derived from the liberated sugar after acid hydrolysis. Compounds 2 and 4 could significantly inhibit the expression of pro-inflammatory iNOS protein at 10 µM. In contrast, 1–3 were found to stimulate the expression of COX-2 protein at this concentration. Steroids 1 and 4 also showed cytotoxicity toward the selected human liver cancer cells. |
| Posted: 16 Feb 2012 12:00 AM PST Probiotics possess potential therapeutic and preventative effects for various diseases and metabolic disorders. One important limitation for the oral delivery of probiotics is the harsh conditions of the upper gastrointestinal tract (GIT) which challenge bacterial viability and activity. One proposed method to surpass this obstacle is the use of microencapsulation to improve the delivery of bacterial cells to the lower GIT. The aim of this study is to use alginate-poly-L-lysine-alginate (APA) microcapsules to encapsulate Lactobacillus fermentum NCIMB 5221 and characterize its enzymatic activity and viability through a simulated GIT. This specific strain, in previous research, was characterized for its inherent ferulic acid esterase (FAE) activity which could prove beneficial in the development of a therapeutic for the treatment and prevention of cancers and metabolic disorders. Our findings demonstrate that the APA microcapsule does not slow the mass transfer of substrate into and that of the FA product out of the microcapsule, while also not impairing bacterial cell viability. The use of simulated gastrointestinal conditions led to a significant 2.5 log difference in viability between the free (1.10 × 104 ± 1.00 × 103 cfu/mL) and the microencapsulated (5.50 × 106 ± 1.00 × 105 cfu/mL) L. fermentum NCIMB 5221 following exposure. The work presented here suggests that APA microencapsulation can be used as an effective oral delivery method for L. fermentum NCIMB 5221, a FAE-active probiotic strain. |
| Pharmaceuticals, Vol. 5, Pages 209-235: Structure Based Antibody-Like Peptidomimetics Posted: 16 Feb 2012 12:00 AM PST Biologics such as monoclonal antibodies (mAb) and soluble receptors represent new classes of therapeutic agents for treatment of several diseases. High affinity and high specificity biologics can be utilized for variety of clinical purposes. Monoclonal antibodies have been used as diagnostic agents when coupled with radionuclide, immune modulatory agents or in the treatment of cancers. Among other limitations of using large molecules for therapy the actual cost of biologics has become an issue. There is an effort among chemists and biologists to reduce the size of biologics which includes monoclonal antibodies and receptors without a reduction of biological efficacy. Single chain antibody, camel antibodies, Fv fragments are examples of this type of deconstructive process. Small high-affinity peptides have been identified using phage screening. Our laboratory used a structure-based approach to develop small-size peptidomimetics from the three-dimensional structure of proteins with immunoglobulin folds as exemplified by CD4 and antibodies. Peptides derived either from the receptor or their cognate ligand mimics the functions of the parental macromolecule. These constrained peptides not only provide a platform for developing small molecule drugs, but also provide insight into the atomic features of protein-protein interactions. A general overview of the reduction of monoclonal antibodies to small exocyclic peptide and its prospects as a useful diagnostic and as a drug in the treatment of cancer are discussed. |
| Posted: 16 Feb 2012 12:00 AM PST Trimethoprim (TMP) is a dihydrofolate reductase (DHFR) inhibitor which prevents the conversion of dihydrofolic acid into tetrahydrofolic acid, resulting in the depletion of the latter and leading to bacterial death. Oral bioavailability of TMP is hindered by both its low solubility and low permeability. This study aims to prepare novel salts of TMP using anionic amino acids; aspartic and glutamic acid as counter ions in order to improve solubility and dissolution. TMP salts were prepared by lyophilisation and characterized using FT-IR spectroscopy, proton nuclear magnetic resonance (1HNMR), Differential Scanning Calorimetry (DSC) and Thermogravimetric analysis (TGA). Both the amino acids formed salts with TMP in a 1:1 molar ratio and showed a 280 fold improvement in solubility. Investigation of the microbiological activity of the prepared salts against TMP sensitive Escherichia coli showed that the new salts not only retained antibacterial activity but also exhibited higher zone of inhibition which was attributed to improved physicochemical characters such as higher solubility and dissolution. The results are an important finding that could potentially impact on faster onset of antibacterial activity and reduced therapeutic dose when administered to patients. Studies are underway investigating the effect of ion-pairing TMP with amino acids on the permeability profile of the drug. |
| Posted: 16 Feb 2012 12:00 AM PST Pegylation using heterotelechelic poly(ethylene glycol) (PEG) offers many possibilities to create high-performance molecules and materials. A versatile route is proposed to synthesize heterobifunctional PEG containing diverse combinations of azide, amine, thioacetate, thiol, pyridyl disulfide, as well as activated hydroxyl end groups. Asymmetric activation of one hydroxyl end group enables the heterobifunctionalization while applying selective monotosylation of linear, symmetrical PEG as a key step. The azide function is introduced by reacting monotosyl PEG with sodium azide. A thiol end group is obtained by reaction with sodium hydrosulfide. The activation of the hydroxyl end group and subsequent reaction with potassium carbonate/thioacetic acid yields a thioacetate end group. The hydrolysis of the thioester end group by ammonia in presence of 2,2′-dipyridyl disulfide provides PEG pyridyl disulfide. Amine terminated PEG is prepared either by reduction of the azide or by nucleophilic substitution of mesylate terminated PEG using ammonia. In all cases, >95% functionalization of the PEG end groups is achieved. The PEG derivatives particularly support the development of materials for biomedical applications. For example, grafting up to 13% of the Na-alg monomer units with α-amine-ω-thiol PEG maintains the gelling capacity in presence of calcium ions but simultaneous, spontaneous disulfide bond formation reinforces the initial physical hydrogel. |
| Posted: 16 Feb 2012 12:00 AM PST Antimicrobial peptides (APs) are an important part of the innate immune system in epithelial and non-epithelial surfaces. So far, many different antimicrobial peptides from various families have been discovered in non-vertebrates and vertebrates. They are characterized by antibiotic, antifungal and antiviral activities against a variety of microorganisms. In addition to their role as endogenous antimicrobials, APs participate in multiple aspects of immunity. They are involved in septic and non-septic inflammation, wound repair, angiogenesis, regulation of the adaptive immune system and in maintaining homeostasis. Due to those characteristics AP could play an important role in many practical applications. Limited therapeutic efficiency of current antimicrobial agents and the emerging resistance of pathogens require alternate antimicrobial drugs. The purpose of this review is to highlight recent literature on functions and mechanisms of APs. It also shows their current practical applications as peptide therapeutics and bioactive polymers and discusses the possibilities of future clinical developments. |
| Resistance 3 OST now available Posted: 15 Feb 2012 08:10 PM PST Soundtrack distributed by Sumthing Else Music Works; on sale at Best Buy, GameStop, and other retail outlets.
 Gamers who enjoyed the sounds and tunes emanating from last year's PS3 exclusive shooter Resistance 3 can now get the recently released original soundtrack in CD form. The Resistance 3 original soundtrack CD is distributed under the Sumthing Else Music Works, which is labelled through a licensing relationship with Sony Computer Entertainment America and game developers Insomniac Games. The game's music is composed by Boris Salchow and is recorded at Abbey Road Studios in London. Interested parties can buy the CD at retail outlets such as Best Buy, GameStop, Walmart, Amazon, and Barnes & Noble. Resistance 3 garnered critical acclaim for its huge arsenal of weapons, gorgeous environmental designs, challenging campaign, and online multiplayer. For more information, check out GameSpot's recent review.
Read and Post Comments | Get the full article at GameSpot "Resistance 3 OST now available" was posted by Jonathan Leo Toyad on Wed, 15 Feb 2012 20:10:11 -0800 |
| The HotSpot - PlayStation Vita - 2/15/12 Posted: 15 Feb 2012 06:40 PM PST After a week of putting the PS Vita through its paces, The HotSpot crew delivers its verdict on Sony's newest handheld.
Get the full article at GameSpot "The HotSpot - PlayStation Vita - 2/15/12" was posted by Tom Magrino on Wed, 15 Feb 2012 18:40:16 -0800 |
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