вторник, 26 февраля 2013 г.

Games

Games

IJMS, Vol. 14, Pages 4613-4628: Covalent Immobilization of Bacillus licheniformis γ-Glutamyl Transpeptidase on Aldehyde-Functionalized Magnetic Nanoparticles

Posted: 26 Feb 2013 12:00 AM PST

This work presents the synthesis and use of surface-modified iron oxide nanoparticles for the covalent immobilization of Bacillus licheniformis γ-glutamyl transpeptidase (BlGGT). Magnetic nanoparticles were prepared by an alkaline solution of divalent and trivalent iron ions, and they were subsequently treated with 3-aminopropyltriethoxysilane (APES) to obtain the aminosilane-coated nanoparticles. The functional group on the particle surface and the amino group of BlGGT was then cross-linked using glutaraldehyde as the coupling reagent. The loading capacity of the prepared nanoparticles for BlGGT was 34.2 mg/g support, corresponding to 52.4% recovery of the initial activity. Monographs of transmission electron microscopy revealed that the synthesized nanoparticles had a mean diameter of 15.1 ± 3.7 nm, and the covalent cross-linking of the enzyme did not significantly change their particle size. Fourier transform infrared spectroscopy confirmed the immobilization of BlGGT on the magnetic nanoparticles. The chemical and kinetic behaviors of immobilized BlGGT are mostly consistent with those of the free enzyme. The immobilized enzyme could be recycled ten times with 36.2% retention of the initial activity and had a comparable stability respective to free enzyme during the storage period of 30 days. Collectively, the straightforward synthesis of aldehyde-functionalized nanoparticles and the efficiency of enzyme immobilization offer wide perspectives for the practical use of surface-bound BlGGT.

IJMS, Vol. 14, Pages 4596-4612: Atypical Protein Phosphatases: Emerging Players in Cellular Signaling

Posted: 26 Feb 2013 12:00 AM PST

It has generally been considered that protein phosphatases have more diverse catalytic domain structures and mechanisms than protein kinases; however, gene annotation efforts following the human genome project appeared to have completed the whole array of protein phosphatases. Ser/Thr phosphatases are divided into three subfamilies that have different structures from each other, whereas Tyr phosphatases and dual-specificity phosphatases targeting Tyr, Ser and Thr belong to a single large family based on their common structural features. Several years of research have revealed, however, the existence of unexpected proteins, designated here as "atypical protein phosphatases", that have structural and enzymatic features different from those of the known protein phosphatases and are involved in important biological processes. In this review, we focus on the identification and functional characterization of atypical protein phosphatases, represented by eyes absent (EYA), suppressor of T-cell receptor signaling (Sts) and phosphoglycerate mutase family member 5 (PGAM5) and discuss their biological significance in cellular signaling.

IJMS, Vol. 14, Pages 4580-4595: Brain Activity of Thioctic Acid Enantiomers: In Vitro and in Vivo Studies in an Animal Model of Cerebrovascular Injury

Posted: 26 Feb 2013 12:00 AM PST

Oxidative stress is an imbalance between the production of free radicals and antioxidant defense mechanisms, potentially leading to tissue damage. Oxidative stress has a key role in the development of cerebrovascular and/or neurodegenerative diseases. This phenomenon is mainly mediated by an enhanced superoxide production by the vascular endothelium with its consequent dysfunction. Thioctic, also known as alpha-lipoic acid (1,2-dithiolane-3-pentanoic acid), is a naturally occurring antioxidant that neutralizes free radicals in the fatty and watery regions of cells. Both the reduced and oxidized forms of the compound possess antioxidant ability. Thioctic acid has two optical isomers designated as (+)- and (−)-thioctic acid. Naturally occurring thioctic acid is the (+)-thioctic acid form, but the synthetic compound largely used in the market for stability reasons is a mixture of (+)- and (−)-thioctic acid. The present study was designed to compare the antioxidant activity of the two enantiomers versus the racemic form of thioctic acid on hydrogen peroxide-induced apoptosis in a rat pheochromocytoma PC12 cell line. Cell viability was evaluated by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and free oxygen radical species (ROS) production was assessed by flow cytometry. Antioxidant activity of the two enantiomers and the racemic form of thioctic acid was also evaluated in spontaneously hypertensive rats (SHR) used as an in vivo model of increased oxidative stress. A 3-h exposure of PC12 cells to hydrogen peroxide (H2O2) significantly decreased cell viability and increased levels of intracellular ROS production. Pre-treatment with racemic thioctic acid or (+)-enantiomer significantly inhibited H2O2-induced decrease in cell viability from the concentration of 50 μmol/L and 20 μmol/L, respectively. Racemic thioctic acid and (+)-salt decreased levels of intracellular ROS, which were unaffected by (−)-thioctic acid. In the brain of SHR, the occurrence of astrogliosis and neuronal damage, with a decreased expression of neurofilament 200 kDa were observed. Treatment of SHR for 30 days with (+)-thioctic acid reduced the size of astrocytes and increased the neurofilament immunoreaction. The above findings could contribute to clarify the role played by thioctic acid in central nervous system injury related to oxidative stress. The more pronounced effect of (+)-thioctic acid observed in this study may have practical therapeutic implications worthy of being investigated in further preclinical and clinical studies.

IJMS, Vol. 14, Pages 4560-4579: Small Regulatory RNAs in the Control of Motility and Biofilm Formation in E. coli and Salmonella

Posted: 26 Feb 2013 12:00 AM PST

Biofilm formation in Escherichia coli and other enteric bacteria involves the inverse regulation of the synthesis of flagella and biofilm matrix components such as amyloid curli fibres, cellulose, colanic acid and poly-N-acetylglucosamine (PGA). Physiologically, these processes reflect the transition from growth to stationary phase. At the molecular level, they are tightly controlled by various sigma factors competing for RNA polymerase, a series of transcription factors acting in hierarchical regulatory cascades and several nucleotide messengers, including cyclic-di-GMP. In addition, a surprisingly large number of small regulatory RNAs (sRNAs) have been shown to directly or indirectly modulate motility and/or biofilm formation. This review aims at giving an overview of these sRNA regulators and their impact in biofilm formation in E. coli and Salmonella. Special emphasis will be put on sRNAs, that have known targets such as the mRNAs of the flagellar master regulator FlhDC, the stationary phase sigma factor σS (RpoS) and the key biofilm regulator CsgD that have recently been shown to act as major hubs for regulation by multiple sRNAs.

Molecules, Vol. 18, Pages 2589-2597: Hirtinone, a Novel Cycloartane-Type Triterpene and Other Compounds from Trichilia hirta L. (Meliaceae)

Posted: 26 Feb 2013 12:00 AM PST

One novel triterpene cycloartane-type, named hirtinone (1), six protolimonoids – nilocitin (2), dihydronilocitin B (3), melianone epimers (4) and (5), piscidinol A (6) and melianone lactone (7), one tertranortriterpenoid, hirtin (8), and one sesquiterpene, spathulenol (9), were identified in the fruits of Trichilia hirta. The structures were established by 1D and 2D NMR (1H and 13C-NMR, DEPTQ, 1H-1H-COSY, 1H-1H-NOESY, HSQC and HMBC), high resolution mass spectroscopy (HR-ESI-MS) and infrared (IR) spectral data.

ComicTagger 1.1.3-beta - Automatically write metadata to comic archives. (Free)

Posted: 26 Feb 2013 02:56 AM PST



ComicTagger is a cross-platform app for writing metadata to comic archives, written in Python and PyQt.

Features:

  • Runs on OS X, Microsoft Windows, and Linux systems
  • Communicates with an online database (Comic Vine) for acquiring metadata
  • Uses image processing to automatically match a given archive with the correct issue data
  • Batch processing in the GUI for tagging hundreds or more comics at a time
  • Reads and writes multiple tagging schemes ( ComicBookLover and ComicRack, with more planned).
  • Reads and writes RAR and Zip (CBR and CBZ) archives (external tools needed for writing RAR)
  • Command line interface (CLI) on all platforms (including Windows), which supports batch operations, and which can be used in native scripts for complex operations.



Version 1.1.3-beta:
  • Fixed a bug when renaming on non-English systems
  • Fixed issue when saving settings on non-English systems
  • Fixed a bug when comic contains non-RGB images
  • Fixed a rare crash when comic image is not-RGB format
  • Fixed sequence order of ComicInfo?.xml items


OS X 10.6.8 or later

Download Now

Vita price needs to follow Japan's lead asap

Posted: 26 Feb 2013 02:56 AM PST

PlayStation Vita has suffered one of the most difficult and trying first years of any console we can remember. Or, to put it more starkly, it's had one of

Nutrients, Vol. 5, Pages 624-636: Selenoprotein-Transgenic Chlamydomonas reinhardtii

Posted: 26 Feb 2013 12:00 AM PST

Selenium (Se) deficiency is associated with the occurrence of many diseases. However, excessive Se supplementation, especially with inorganic Se, can result in toxicity. Selenoproteins are the major forms of Se in vivo to exert its biological function. Expression of those selenoproteins, especially with the application of a newly developed system, is thus very important for studying the mechanism of Se in nutrition. The use of Chlamydomonas reinhardtii (C. reinhardtii) as a biological vector to express an heterogeneous protein is still at the initial stages of development. In order to investigate the possibility of using this system to express selenoproteins, human 15-KDa selenoprotein (Sep15), a small but widely distributed selenoprotein in mammals, was chosen for the expression platform test. Apart from the wild-type human Sep15 gene fragment, two Sep15 recombinants were constructed containing Sep15 open reading frame (ORF) and the selenocysteine insertion sequence (SECIS) element from either human Sep15 or C. reinhardtii selenoprotein W1, a highly expressed selenoprotein in this alga. Those Sep15-containing plasmids were transformed into C. reinhardtii CC-849 cells. Results showed that Sep15 fragments were successfully inserted into the nuclear genome and expressed Sep15 protein in the cells. The transgenic and wild-type algae demonstrated similar growth curves in low Se culture medium. To our knowledge, this is the first report on expressing human selenoprotein in green alga.

Religions, Vol. 4, Pages 132-144: The Role of the Meaning of Life and Religious Experience of God’s Presence and God's Absence Amongst Students with Different Levels of Conscience Sensitivity

Posted: 26 Feb 2013 12:00 AM PST

The aim of the author's own research was: (a) defining the level of meaning in life and the level of religious experience (God's presence and God's absence) in groups of students with high and low levels of conscience sensitivity and (b) showing the connection between meaning in life and the level of religious experience (God's presence and God's absence) in groups of students with high and low levels of conscience sensitivity. The study was conducted in 2009–2010 among university students in Kraków. The subject group consisted of students of several non-Catholic public and state universities. All participants were Polish born, culturally homogeneous, and stemmed from families of average affluence. The age of the respondents ranged from 21 to 25. Two-hundred and forty sets of correctly completed questionnaires were used for the results analysis.

Applied Sciences, Vol. 3, Pages 168-188: Linear Electro Optic Effect for High Repetition Rate Carrier Envelope Phase Control of Ultra Short Laser Pulses

Posted: 26 Feb 2013 12:00 AM PST

This paper is devoted to analyzing the principle and applications of the linear electro-optic (EO) effect for the control of the carrier-envelope-phase (CEP). We introduce and detail here an original method, which relies on the use of an EO dispersive prism pair in a compressor-like configuration. We show that, by choosing an adequate geometry, it is possible to shift the CEP without changing the group delay (isochronous carrier-envelope-phase shifter) or change the induced group delay without varying the CEP. According to our calculations, when applying an electric field around 400 V/cm to the rubidium titanyle phosphate (RTP) prisms in a double pass configuration (2 × 40 mm total length), one obtains a CEP shift of π rad at 800 nm without inducing a group delay. In contrast, this CEP shift is obtained for an electric field around 1.4 kV/cm in a RTP rectangular slab of the same total length and, in this case, the group delay is of the order of a few fs.

Marine Drugs, Vol. 11, Pages 611-622: Thalassospiramide G, a New γ-Amino-Acid-Bearing Peptide from the Marine Bacterium Thalassospira sp.

Posted: 26 Feb 2013 12:00 AM PST

In the chemical investigation of marine unicellular bacteria, a new peptide, thalassospiramide G (1), along with thalassospiramides A and D (2–3), was discovered from a large culture of Thalassospira sp. The structure of thalassospiramide G, bearing γ-amino acids, such as 4-amino-5-hydroxy-penta-2-enoic acid (AHPEA), 4-amino-3,5-dihydroxy-pentanoic acid (ADPA), and unique 2-amino-1-(1H-indol-3-yl) ethanone (AIEN), was determined via extensive spectroscopic analysis. The absolute configuration of thalassospiramide D (3), including 4-amino-3-hydroxy-5-phenylpentanoic acid (AHPPA), was rigorously determined by 1H–1H coupling constant analysis and chemical derivatization. Thalassospiramides A and D (2–3) inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated mouse macrophage RAW 264.7 cells, with IC50 values of 16.4 and 4.8 μM, respectively.

Marine Drugs, Vol. 11, Pages 599-610: Eleganolone, a Diterpene from the French Marine Alga Bifurcaria bifurcata Inhibits Growth of the Human Pathogens Trypanosoma brucei and Plasmodium falciparum

Posted: 26 Feb 2013 12:00 AM PST

Organic extracts of 20 species of French seaweed have been screened against Trypanosoma brucei rhodesiense trypomastigotes, the parasite responsible for sleeping sickness. These extracts have previously shown potent antiprotozoal activities in vitro against Plasmodium falciparum and Leishmania donovani. The selectivity of the extracts was also evaluated by testing cytotoxicity on a mammalian L6 cell line. The ethyl acetate extract of the brown seaweed, Bifurcaria bifurcata, showed strong trypanocidal activity with a mild selectivity index (IC50 = 0.53 µg/mL; selectivity index (SI) = 11.6). Bio-guided fractionation led to the isolation of eleganolone, the main diterpenoid isolated from this species. Eleganolone contributes only mildly to the trypanocidal activity of the ethyl acetate extract (IC50 = 45.0 µM, SI = 4.0). However, a selective activity against P. falciparum erythrocytic stages in vitro has been highlighted (IC50 = 7.9 µM, SI = 21.6).

Marine Drugs, Vol. 11, Pages 584-598: Characterization of Palytoxin Binding to HaCaT Cells Using a Monoclonal Anti-Palytoxin Antibody

Posted: 26 Feb 2013 12:00 AM PST

Palytoxin (PLTX) is the reference compound for a group of potent marine biotoxins, for which the molecular target is Na+/K+-ATPase. Indeed, ouabain (OUA), a potent blocker of the pump, is used to inhibit some PLTX effects in vitro. However, in an effort to explain incomplete inhibition of PLTX cytotoxicity, some studies suggest the possibility of two different binding sites on Na+/K+-ATPase. Hence, this study was performed to characterize PLTX binding to intact HaCaT keratinocytes and to investigate the ability of OUA to compete for this binding. PLTX binding to HaCaT cells was demonstrated by immunocytochemical analysis after 10 min exposure. An anti-PLTX monoclonal antibody-based ELISA showed that the binding was saturable and reversible, with a Kd of 3 × 10−10 M. However, kinetic experiments revealed that PLTX binding dissociation was incomplete, suggesting an additional, OUA-insensitive, PLTX binding site. Competitive experiments suggested that OUA acts as a negative allosteric modulator against high PLTX concentrations (0.3–1.0 × 10−7 M) and possibly as a non-competitive antagonist against low PLTX concentrations (0.1–3.0 × 10−9 M). Antagonism was supported by PLTX cytotoxicity inhibition at OUA concentrations that displaced PLTX binding (1 × 10−5 M). However, this inhibition was incomplete, supporting the existence of both OUA-sensitive and -insensitive PLTX binding sites.

Marine Drugs, Vol. 11, Pages 581-583: Marine Drugs Best Paper Award 2013

Posted: 26 Feb 2013 12:00 AM PST

To recognize the most outstanding papers in the area of research, development and production of drugs from the sea, including marine natural product chemistry, that have been published in Marine Drugs, we would like to start an annual "Best Paper Award".

IJERPH, Vol. 10, Pages 808-815: Outbreak of Ampicillin/Piperacillin-Resistant Klebsiella Pneumoniae in a Neonatal Intensive Care Unit (NICU): Investigation and Control Measures

Posted: 26 Feb 2013 12:00 AM PST

Klebsiella pneumoniae is a frequent cause of infectious outbreaks in Neonatal Intensive Care Units (NICUs). The aim of this paper is to describe an outbreak occurred in a 13-bed NICU and the control measures adopted in order to interrupt the chain of transmission. We described the microbiological investigations, the NICU staff compliance to the infection control measures by means of a specifically designed check-list and the control measures adopted. Six cases of primary bloodstream infections sustained by ampicillin/piperacillin-resistant Klebsiella pneumoniae were observed over a two-month period. One culture obtained from a 12% saccarose multiple-dose solution allowed the growth of Klebsiella pneumoniae. During the inspections performed by the Hospital Infection Control Team, using the check-list for the evaluation of the NICU staff compliance to the infection control measures, several breaches in the infection control policy were identified and control measures were adopted. In our case the definition of a specific check-list led to the adoption of the correct control measures. Further studies would be helpful in order to develop a standard check-list able to identify critical flows in the adhesion to the guidelines. It could be used in different NICUs and allow to obtain reproducible levels of infection control.

IJERPH, Vol. 10, Pages 793-807: Heavy Metal Pollution of Lakes along the Mid-Lower Reaches of the Yangtze River in China: Intensity, Sources and Spatial Patterns

Posted: 26 Feb 2013 12:00 AM PST

Lakes in the middle and lower reaches of the Yangtze River form a shallow lake group unique in the World that is becoming increasingly polluted by heavy metals. Previous studies have largely focused on individual lakes, with limited exploration of the regional pattern of heavy metal pollution of the lake group in this area. This paper explores the sources, intensity and spatial patterns of heavy metal pollution of lake sediments. A total of 45 sample lakes were selected and the concentrations of key metal elements in the sediments of each lake were measured. The cluster analysis (CA), principal component analysis (PCA) and Geo-accumulation index (Ig) analysis permitted analysis of the source and pollution intensity of the target lakes. Results suggested a notable spatial variation amongst the sample lakes. Lakes in the upper part of the lower reach of the Yangtze River surrounded by typical urban landscapes were strongly or extremely polluted, with high concentrations of Pb, Zn, Cu and Cd in their sediments. This was attributed to large amount of untreated industrial discharges and municipal sewage produced within the lake catchments. In contrast, the heavy-metal pollution of lakes in the Taihu Delta area was notably lower due to industrial restructuring and implementation of effective environmental protection measures. Lakes along the middle reach of Yangtze River surrounded by agricultural areas were unpolluted to moderately polluted by heavy metals overall. Our results suggested that lakes in the central part of China require immediate attention and efforts should be made to implement management plans to prevent further degradation of water quality in these lakes.

IJERPH, Vol. 10, Pages 776-792: Does Farming Have an Effect on Health Status? A Comparison Study in West Greece

Posted: 26 Feb 2013 12:00 AM PST

Investigating the health status of agricultural workers is a challenging goal. Contradictory outcomes concerning farmers' health are reported in the literature. In this cross-sectional study, certain clinical and neurobehavioral health outcomes were compared between farmers and non-farmers living in the same rural area. Farmers (328) and non-farmers (347), matched per age and sex, were selected randomly in an agricultural area in West Greece. Both groups underwent haematological and biochemical examinations and were administered two neurobehavioral tests, namely the Mini-Mental State Examination (MMSE) and the Montgomery-Åsberg Depression Rating Scale (MADRS). Sociodemographic, personal medical, nutritional and lifestyle data were recorded. According to personal statements, farmers suffered from hypertension, cardiovascular, orthopaedic and ENT problems in higher frequency. Haematocrit, haemoglobin and serum cholinesterase's activity were found to be lower among farmers. Lower prevalence of hypertension and better performances on MMSE and MADRS tests were recorded in young farmers in relation to young non-farmers, while these findings were reversed in older ages. Odds Ratios were calculated through multivariate logistic regression models. Factors affecting these impairments remain to be clarified.

Remote Sensing, Vol. 5, Pages 1001-1023: Impacts of Spatial Variability on Aboveground Biomass Estimation from L-Band Radar in a Temperate Forest

Posted: 26 Feb 2013 12:00 AM PST

Estimation of forest aboveground biomass (AGB) has become one of the main challenges of remote sensing science for global observation of carbon storage and changes in the past few decades. We examine the impact of plot size at different spatial resolutions, incidence angles, and polarizations on the forest biomass estimation using L-band polarimetric Synthetic Aperture Radar data acquired by NASA's Unmanned Aerial Vehicle Synthetic Aperture Radar (UAVSAR) airborne system. Field inventory data from 32 1.0 ha plots (AGB < 200 Mg ha−1) in approximately even-aged forests in a temperate to boreal transitional region in the state of Maine were divided into subplots at four different spatial scales (0.0625 ha, 0.25 ha, 0.5 ha, and 1.0 ha) to quantify aboveground biomass variations. The results showed a large variability in aboveground biomass at smaller plot size (0.0625 ha). The variability decreased substantially at larger plot sizes (>0.5 ha), suggesting a stability of field-estimated biomass at scales of about 1.0 ha. UAVSAR backscatter was linked to the field estimates of aboveground biomass to develop parametric equations based on polarized returns to accurately map biomass over the entire radar image. Radar backscatter values at all three polarizations (HH, VV, HV) were positively correlated with field aboveground biomass at all four spatial scales, with the highest correlation at the 1.0 ha scale. Among polarizations, the cross-polarized HV had the highest sensitivity to field estimated aboveground biomass (R2 = 0.68). Algorithms were developed that combined three radar backscatter polarizations (HH, HV, and VV) to estimate aboveground biomass at the four spatial scales. The predicted aboveground biomass from these algorithms resulted in decreasing estimation error as the pixel size increased, with the best results at the 1 ha scale with an R2 of 0.67 (p < 0.0001), and an overall RMSE of 44 Mg·ha−1. For AGB < 150 Mg·ha−1, the error reduced to 23 Mg·ha−1 (±15%), suggesting an improved AGB prediction below the L-band sensitivity range to biomass. Results also showed larger bias in aboveground biomass estimation from radar at smaller scales that improved at larger spatial scales of 1.0 ha with underestimation of −3.62 Mg·ha−1 over the entire biomass range.

Materials, Vol. 6, Pages 713-725: Structural and Luminescence Properties of Lu2O3:Eu3+ F127 Tri-Block Copolymer Modified Thin Films Prepared by Sol-Gel Method

Posted: 26 Feb 2013 12:00 AM PST

Lu2O3:Eu3+ transparent, high density, and optical quality thin films were prepared using the sol-gel dip-coating technique, starting with lutetium and europium nitrates as precursors and followed by hydrolysis in an ethanol-ethylene glycol solution. Acetic acid and acetylacetonate were incorporated in order to adjust pH and as a sol stabilizer. In order to increment the thickness of the films and orient the structure, F127 Pluronic acid was incorporated during the sol formation. Structural, morphological, and optical properties of the films were investigated for different F127/Lu molar ratios (0–5) in order to obtain high optical quality films with enhanced thickness compared with the traditional method. X-ray diffraction (XRD) shows that the films present a highly oriented cubic structure <111> beyond 1073 K for a 3-layer film, on silica glass substrates. The thickness, density, porosity, and refractive index evolution of the films were investigated by means of m-lines microscopy along with the morphology by scanning electron microscope (SEM) and luminescent properties.

Molecules, Vol. 18, Pages 2587-2588: Lee et al., Inhibitory Effect and Mechanism of Antiproliferation of Isoatriplicolide Tiglate (PCAC) from Paulownia Coreana. Molecules 2012, 17, 5945-5951: A Note Regarding Paulownia coreana.

Posted: 26 Feb 2013 12:00 AM PST

I read with interest the article by Lee et al. entitled "Inhibitory Effect and Mechanism of Antiproliferation of Isoatriplicolide Tiglate (PCAC) from Paulownia Coreana" [1]. This article is quite interesting and the authors should be complimented for the significant amount of work they have done. The purpose of this letter is to call attention to the need for some clarification on the name of the plant described in this article. Lee et al. state: "Paulownia coreana has traditionally been used as the medicine and health food in the treatment of cancer and infectious diseases." and elsewhere: "In fact, many cancer research studies have been conducted using traditional medicinal plants such as P. coreana." [1].
Автор: на

Комментариев нет:

Отправить комментарий

Подписаться на: Комментарии к сообщению (Atom)