четверг, 21 июня 2012 г.

Games

Games

DROD: King Dugan's Dungeon Lite - Episode 3

Posted: 20 Jun 2012 07:58 PM PDT

Platform: Flash — DROD: King Dugan's Dungeon Lite - Episode 3 Beethro Budkin has had to face all sorts of challenges crawling through the first eight floors of Dugan's dungeon. Now though, he stands on the precipice of levels nine through twelve, and they're filled with Deadly Rooms of Death of all kinds! Caravel Games' series of turn-based puzzle-strategy games gets just a bit more hair-pulling in DROD: King Dugan's Dungeon Lite - Episode 3. Tagged as: browser, caravelgames, drod, dungeoncrawler, flash, free, game, linux, mac, puzzle, rating-o, retro, roguelike, rpg, strategy, topdown, turnbased, windows

Transcripted - Videos and Trailers

Posted: 21 Jun 2012 02:51 AM PDT

Transcripted first trailer.

Scotland Yard - Videos and Trailers

Posted: 21 Jun 2012 01:49 AM PDT

Scotland Yard trailer.

StarBunker:Guardians2 HD - Videos and Trailers

Posted: 21 Jun 2012 02:39 AM PDT

StarBunker:Guardians2 HD trailer.

Smite - Videos and Trailers

Posted: 21 Jun 2012 02:23 AM PDT

Smite tutorial video.

Okami HD - Videos and Trailers

Posted: 21 Jun 2012 01:09 AM PDT

Okami HD first trailer.

Magic: The Gathering - Duels of the Planeswalkers 2013 - Videos and Trailers

Posted: 21 Jun 2012 01:25 AM PDT

Magic: The Gathering - Duels of the Planeswalkers 2013 gamepley trailer.

Summer Stars - Videos and Trailers

Posted: 21 Jun 2012 02:46 AM PDT

Summer Stars trailer.

Magic: The Gathering Faithfuls Rewarded With Free Deck

Posted: 21 Jun 2012 03:02 AM PDT

Wizards of the Coast is offering a nice little bonus for returning players of Magic: The Gathering , granting access to a free deck of cards in Duels of the Planeswalkers 2013 Joystiq's David Hinkle noticed the digital gift when first booting up his review copy of the latest duel-a-thon for PlayStation 3, Xbox 360 and PC.

This posting includes an audio/video/photo media file: Download Now

Tor anonymity developers tell all

Posted: 21 Jun 2012 02:50 AM PDT

Runa from the Tor anonymity project sez, "Karen and I will be answering questions on Reddit today. Feel free to ask us anything you'd like relating to Tor and the Tor Project!"

Tiny Dragon Dress Up

Posted: 21 Jun 2012 01:54 AM PDT

Raise and dress up your own magical dragon! Dress him, add items and make him the most beautiful monster in the world.

IJMS, Vol. 13, Pages 7776-7787: Polydatin Attenuates Hypoxic Pulmonary Hypertension and Reverses Remodeling through Protein Kinase C Mechanisms

Posted: 21 Jun 2012 12:00 AM PDT

Hypoxic pulmonary hypertension is a life-threatening emergency if untreated. Consistent pulmonary hypertension also leads to arteries and ventricular remodeling. The clinical therapeutic strategy for pulmonary hypertension and the corresponding remodeling mainly interacts with NO, angiotensin II (Ang II) and elevated endothelin (ET) targets. In the present study, we evaluated the effects of polydatin on hypoxia-induced pulmonary hypertension. It was observed that polydatin attenuated hypoxic pulmonary hypertension, reversed remodeling, and regulated NO, Ang II, ET contents in the serum and lung samples. However, forced activation of PKC signaling by its selective activator thymeleatoxin (THX) could abate the effects of polydatain. These results suggest that polydatin might be a promising candidate for hypoxic pulmonary treatment through interaction with PKC mechanisms.

IJMS, Vol. 13, Pages 7762-7775: Preparation of Porous Scaffolds from Silk Fibroin Extracted from the Silk Gland of Bombyx mori (B. mori)

Posted: 21 Jun 2012 12:00 AM PDT

In order to use a simple and ecofriendly method to prepare porous silk scaffolds, aqueous silk fibroin solution (ASF) was extracted from silk gland of 7-day-old fifth instar larvae of Bombyx mori (B. mori). SDS-page analysis indicated that the obtained fibroin had a molecular weight higher than 200 kDa. The fabrication of porous scaffolds from ASF was achieved by using the freeze-drying method. The pore of porous scaffolds is homogenous and tends to become smaller with an increase in the concentration of ASF. Conversely, the porosity is decreased. The porous scaffolds show impressive compressive strength which can be as high as 6.9 ± 0.4 MPa. Furthermore, ASF has high cell adhesion and growth activity. It also exhibits high ALP activity. This implies that porous scaffolds prepared from ASF have biocompatibility. Therefore, the porous scaffolds prepared in this study have potential application in tissue engineering due to the impressive compressive strength and biocompatibility.

IJMS, Vol. 13, Pages 7739-7761: Clinical Neuroprotective Drugs for Treatment and Prevention of Stroke

Posted: 21 Jun 2012 12:00 AM PDT

Stroke is an enormous public health problem with an imperative need for more effective therapies. In therapies for ischemic stroke, tissue plasminogen activators, antiplatelet agents and anticoagulants are used mainly for their antithrombotic effects. However, free radical scavengers, minocycline and growth factors have shown neuroprotective effects in the treatment of stroke, while antihypertensive drugs, lipid-lowering drugs and hypoglycemic drugs have shown beneficial effects for the prevention of stroke. In the present review, we evaluate the treatment and prevention of stroke in light of clinical studies and discuss new anti-stroke effects other than the main effects of drugs, focusing on optimal pharmacotherapy.

IJMS, Vol. 13, Pages 7710-7738: Effects of Methylmercury Contained in a Diet Mimicking the Wayana Amerindians Contamination through Fish Consumption: Mercury Accumulation, Metallothionein Induction, Gene Expression Variations, and Role of the Chemokine CCL2

Posted: 21 Jun 2012 12:00 AM PDT

Methylmercury (MeHg) is a potent neurotoxin, and human beings are mainly exposed to this pollutant through fish consumption. We addressed the question of whether a diet mimicking the fish consumption of Wayanas Amerindians from French Guiana could result in observable adverse effects in mice. Wayanas adult men are subjected to a mean mercurial dose of 7 g Hg/week/kg of body weight. We decided to supplement a vegetarian-based mice diet with 0.1% of lyophilized Hoplias aimara fish, which Wayanas are fond of and equivalent to the same dose as that afflicting the Wayanas Amerindians. Total mercury contents were 1.4 ± 0.2 and 5.4 ± 0.5 ng Hg/g of food pellets for the control and aimara diets, respectively. After 14 months of exposure, the body parts and tissues displaying the highest mercury concentration on a dry weight (dw) basis were hair (733 ng/g) and kidney (511 ng/g), followed by the liver (77 ng/g). Surprisingly, despite the fact that MeHg is a neurotoxic compound, the brain accumulated low levels of mercury (35 ng/g in the cortex). The metallothionein (MT) protein concentration only increased in those tissues (kidney, muscles) in which MeHg demethylation had occurred. This can be taken as a molecular sign of divalent mercurial contamination since only Hg2+ has been reported yet to induce MT accumulation in contaminated tissues. The suppression of the synthesis of the chemokine CCL2 in the corresponding knockout (KO) mice resulted in important changes in gene expression patterns in the liver and brain. After three months of exposure to an aimara-containing diet, eight of 10 genes selected (Sdhb, Cytb, Cox1, Sod1, Sod2, Mt2, Mdr1a and Bax) were repressed in wild-type mice liver whereas none presented a differential expression in KO Ccl2−/− mice. In the wild-type mice brain, six of 12 genes selected (Cytb, Cox1, Sod1, Sod2, Mdr1a and Bax) presented a stimulated expression, whereas all remained at the basal level of expression in KO Ccl2−/− mice. In the liver of aimara-fed mice, histological alterations were observed for an accumulated mercury concentration as low as 32 ng/g, dw, and metal deposits were observed within the cytoplasm of hepatic cells.

Metal Gear Solid 5 confirmed - Report

Posted: 20 Jun 2012 09:15 PM PDT

Hideo Kojima has reportedly announced that the fifth entry in the series will use in-house FOX Engine and conversation trees; date and platforms remain unspecified.

 

Metal Gear Solid creator Hideo Kojima has reportedly confirmed that there will be a fifth entry in the main franchise.

According to an interview conducted with IG Magazine (via Gamekyo.com and Escapist.com), Metal Gear Solid 5 will be running on the Fox Engine, an in-house engine created by Kojima Productions.

Kojima reportedly said that the title will contain more infiltration and possible conversation trees, to further the game's narrative.

"I loved the idea of the social interactions in Deus Ex: Human Revolution that we've seen," Kojima was quoted as saying. "And, if I say more about it, the ninja PR woman behind the door will chop me into pieces."

The sequel will not have direct ties with upcoming Metal Gear Rising: Revengeance. He also confirmed that Metal Gear's main character Solid Snake will be around for the fifth game, despite wanting him to die at the end of Metal Gear Solid 4.

No other details on the game have surfaced, including release date and platforms, and no word on whether an official announcement or teaser will be made and presented at this year's Tokyo Game Show in September.

For more information on the Metal Gear spin-off, Metal Gear Rising: Revengeance, check out GameSpot's recent article.

Read and Post Comments | Get the full article at GameSpot

"Metal Gear Solid 5 confirmed - Report" was posted by Jonathan Toyad on Wed, 20 Jun 2012 21:15:48 -0700

IJMS, Vol. 13, Pages 7694-7709: The Role of PPARα in Metformin-Induced Attenuation of Mitochondrial Dysfunction in Acute Cardiac Ischemia/Reperfusion in Rats

Posted: 21 Jun 2012 12:00 AM PDT

Metformin, an anti-diabetic drug, exerts cardioprotection against ischemia-reperfusion (IR) through the activation of AMPK. However, the molecular mechanisms underlying these beneficial effects remain elusive. In this study, we examined the role of PPARα in mediating cardioprotective effects of metformin on mitochondria. Hearts of male Sprague-Dawley rats perfused by Langendorff were subjected to IR in the presence or absence of metformin and the PPARα inhibitor, GW6471. IR reduced cardiac function and compromised the structural integrity of cardiac cells evidenced by increased LDH release from the hearts. In addition, IR induced mitochondrial dysfunction as evidenced by reduced respiration and increased mitochondrial permeability transition pore (PTP) opening. However, metformin-treated hearts demonstrated improved post-ischemic recovery of cardiac function and reduced cell death that were associated with increased state 3 respiration at complexes I and II (by 27% and 32%, respectively, both p < 0.05) and decreased PTP opening (by 27%, p < 0.05) compared to untreated hearts. The protective effects of metformin on cardiac function and mitochondria were blocked by GW6471. Thus, our results demonstrate that inhibition of PPARα attenuates the beneficial effects of metformin on mitochondria in acute IR.

IJMS, Vol. 13, Pages 7676-7693: Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis

Posted: 21 Jun 2012 12:00 AM PDT

Multiple Sclerosis (MS) is a chronic disease, but in rare fulminant cases rapid progression may lead to death shortly after diagnosis. Currently there is no diagnostic test to predict disease course. The aim of this study was to identify potential biomarkers/proteins related to rapid progression. We present the case history of a 15-year-old male MS patient. Cerebrospinal fluid (CSF) was taken at diagnosis and at the time of rapid progression leading to the patient's death. Using isobaric tag labeling and nanoflow liquid chromatography in conjunction with matrix assisted laser desorption/ionization time of flight tandem mass spectrometry we quantitatively analyzed the protein content of two CSF samples from the patient with fulminant MS as well as one relapsing-remitting (RR) MS patient and one control headache patient, whose CSF analysis was normal. Seventy-eight proteins were identified and seven proteins were found to be more abundant in both fulminant MS samples but not in the RR MS sample compared to the control. These proteins are involved in the immune response, blood coagulation, cell proliferation and cell adhesion. In conclusion, in this pilot study we were able to show differences in the CSF proteome of a rapidly progressing MS patient compared to a more typical clinical form of MS and a control subject.

IJMS, Vol. 13, Pages 7663-7675: Expression of Sox2 and Oct4 and Their Clinical Significance in Human Non-Small-Cell Lung Cancer

Posted: 21 Jun 2012 12:00 AM PDT

Sox2 and Oct4 are transcription factors with the characteristics of regulating self-renewal and differentiation of embryonic stem cell. The aim of this study was to detect the expression of Sox2 and Oct4 and analyze their clinical significance in human non-small-cell lung cancer (NSCLC). Expression of Sox2 and Oct4 were assayed in cancer tissues and their corresponding paracancerous tissues from 44 patients with NSCLC and 21 patients with benign tumors using immunohistochemistry, Western blot, reverse transcription polymerase chain reaction (RT-PCR). The correlation between the expression of Sox2 and Oct4 and tumor type, grade and prognosis and the utility of the two genes in discriminating between benign and malignant tumors were analyzed as well. The results showed that Sox2 and Oct4 positive staining was only seen in the nuclei of cancer cells but not in either the precancerous tissues or benign tumor tissues by immunohistochemistry (p < 0.01). Furthermore, in the lung cancer tissue, the positive rate for Sox2 and Oct4 was 70.5% and 54.5%, respectively. Meanwhile, clinicopathological correlations showed that the Oct4 expression level was significantly associated with poorer differentiation and higher TNM stage of the cancer (p < 0.05). Western blot and RT-PCR analysis showed similar results to immunohistochemistry. Follow-up analysis revealed that expression of Oct4 was significantly associated with poor prognosis of lung cancer. The conclusion is that Sox2 and Oct4 may act as the promising unit markers in directing NSCLC diagnosis and therapy. Also, Oct4 can be regarded as a novel predictor of poor prognosis for NSCLC patients undergoing resection.

IJMS, Vol. 13, Pages 7648-7662: Supercritical Fluid Extraction of Eucalyptus globulus Bark—A Promising Approach for Triterpenoid Production

Posted: 21 Jun 2012 12:00 AM PDT

Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids). In this work, the supercritical fluid extraction (SFE) of Eucalyptus globulus deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100–200 bar), co-solvent (ethanol) content (0, 5 and 8% wt), and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by several tens of bar. At 160 bar and 40 °C, the introduction of 8% (wt) of ethanol greatly improves the yield of triterpenoids more than threefold.
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